Rat BNP 45 ELISA Kit (ab108816)
Key features and details
- Sensitivity: 0.03 ng/ml
- Range: 0.0313 ng/ml - 1 ng/ml
- Sample type: Cell culture supernatant, Plasma, Serum, Tissue Extracts
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Rat
Overview
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Product name
Rat BNP 45 ELISA Kit -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Overall 4.9% Inter-assay Sample n Mean SD CV% Overall 7.1% -
Sample type
Cell culture supernatant, Serum, Plasma, Tissue Extracts -
Assay type
Sandwich (quantitative) -
Sensitivity
= 0.03 ng/ml -
Range
0.031 ng/ml - 1 ng/ml -
Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Rat -
Product overview
Abcam’s BNP 45 rat in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of BNP 45 in plasma, serum, tissue extract, and cell culture supernatants.
A BNP 45 specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently a BNP 45 specific biotinylated detection antibody is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Conjugate is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of BNP 45 captured in plate.
The entire kit may be stored at -20°C for long term storage before reconstitution - Avoid repeated freeze-thaw cycles.
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Notes
There is significant cross-reactivity between BNP32 and BNP45.
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Platform
Microplate
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 1 x 96 tests 100X Streptavidin-Peroxidase Conjugate 1 x 80µl 10X Diluent N Concentrate 1 x 30ml 20X Wash Buffer Concentrate 2 x 30ml 70X Biotinylated Rat BNP 45 Antibody 1 x 90µl BNP 45 Microplate (12 x 8 well strips) 1 unit BNP 45 Standard (Lyophilized) 1 vial Chromogen Substrate 1 x 7ml Sealing Tapes 3 units Stop Solution 1 x 11ml -
Research areas
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Function
Cardiac hormone which may function as a paracrine antifibrotic factor in the heart. Also plays a key role in cardiovascular homeostasis through natriuresis, diuresis, vasorelaxation, and inhibition of renin and aldosterone secretion. Specifically binds and stimulates the cGMP production of the NPR1 receptor. Binds the clearance receptor NPR3. -
Tissue specificity
Brain and also in atria, but at much lower levels than ANP. -
Sequence similarities
Belongs to the natriuretic peptide family. -
Cellular localization
Secreted. - Information by UniProt
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Alternative names
- ANFB_HUMAN
- BNP(1-32)
- BNP(5-29)
see all -
Database links
Images
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Sandwich ELISA - BNP45 Rat (ab108816)
Standard curve : mean of duplicates (+/-SD) with background readings subtracted
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Sandwich ELISA - BNP 45 Rat ELISA Kit (ab108816)Rat BNP45 measured in biological fluids showing quantity (pg) per mL of tested sample