DHEA-S ELISA Kit (ab178642)
Key features and details
- Sensitivity: 0.05 ng/ml
- Range: 0.2 ng/ml - 12 ng/ml
- Sample type: Saliva
- Detection method: Colorimetric
- Assay type: Competitive
- Reacts with: Human
Overview
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Product name
DHEA-S ELISA Kit
See all DHEA-S kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Overall 14 Inter-assay Sample n Mean SD CV% Overall 9 -
Sample type
Saliva -
Assay type
Competitive -
Sensitivity
0.05 ng/ml -
Range
0.2 ng/ml - 12 ng/ml -
Recovery
108.86 %
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Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Human -
Product overview
Abcam’s Dehydroepiandrosterone sulfate (DHEA-S) in vitro competitive ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate quantitative measurement of DHEA-S in saliva.
A 96-well plate has been precoated with anti-DHEA-S antibodies. Samples and the DHEA-S-HRP conjugate are added to the wells, where DHEA-S in the sample competes with the added DHEA-S-HRP conjugate for antibody binding. After incubation, the wells are washed to remove unbound material and TMB substrate is then added which is catalyzed by HRP to produce blue coloration. The reaction is terminated by addition of Stop Solution which stops the color development and produces a color change from blue to yellow. The intensity of signal is inversely proportional to the amount of DHEA-S in the sample and the intensity is measured at 450 nm.
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Platform
Microplate
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 50X Washing Solution 1 x 20ml Anti-DHEA-S IgG Coated Microplate (12 x 8 wells) 1 unit Cover Foil 1 unit DHEA-S High Control 1 x 1ml DHEA-S HRP Conjugate 1 x 1ml DHEA-S Low Control 1 x 1ml DHEA-S Standard 0 - 0 ng/mL 1 x 1ml DHEA-S Standard 1 - 0.2 ng/mL 1 x 1ml DHEA-S Standard 2 - 1.0 ng/mL 1 x 1ml DHEA-S Standard 3 - 3.0 ng/mL 1 x 1ml DHEA-S Standard 4 - 12.0 ng/mL 1 x 1ml Incubation Buffer 1 x 30ml Stop Solution 1 x 15ml Strip holder 1 unit TMB Substrate Solution 1 x 15ml -
Research areas
Images
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Typical Standard Curve
Representative standard curve using ab178642.
