Mouse IGF2 ELISA Kit (ab100696)
Key features and details
- Sensitivity: 1.5 ng/ml
- Range: 2.3 ng/ml - 150 ng/ml
- Sample type: Cell culture supernatant
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Mouse
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 1 x 96 tests 1,000X HRP-Streptavidin Concentrate 1 x 200µl 20X Wash Buffer Concentrate 1 x 25ml 5X Assay Diluent 1 x 15ml Biotinylated anti-Mouse IGF2 (lyophilized) 2 vials IGF2 Microplate (12 x 8 wells) 1 unit Recombinant Mouse IGF2 Standard (lyophilized) 2 vials Stop Solution 1 x 8ml TMB One-Step Substrate Reagent 1 x 12ml -
Research areas
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Function
The insulin-like growth factors possess growth-promoting activity. In vitro, they are potent mitogens for cultured cells. IGF-II is influenced by placental lactogen and may play a role in fetal development.
Preptin undergoes glucose-mediated co-secretion with insulin, and acts as physiological amplifier of glucose-mediated insulin secretion. Exhibits osteogenic properties by increasing osteoblast mitogenic activity through phosphoactivation of MAPK1 and MAPK3. -
Involvement in disease
Epigenetic changes of DNA hypomethylation in IGF2 are a cause of Silver-Russell syndrome (SIRS) [MIM:180860]. SIRS is a clinically heterogeneous condition characterized by severe intrauterine growth retardation, poor postnatal growth, craniofacial features such as a triangular shaped face and a broad forehead, body asymmetry, and a variety of minor malformations. -
Sequence similarities
Belongs to the insulin family. -
Post-translational
modificationsO-glycosylated with a core 1 or possibly core 8 glycan. -
Cellular localization
Secreted. - Information by UniProt
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Alternative names
- C11orf43
- IGF 2
- IGF II
see all -
Database links
- Entrez Gene: 16002 Mouse
- SwissProt: P09535 Mouse
- Unigene: 3862 Mouse
Images
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Standard Curve for Mouse IGF2
Typical standard curve for ab100696
