Anti-SMC3 antibody (ab89618)
Key features and details
- Mouse polyclonal to SMC3
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-SMC3 antibody
See all SMC3 primary antibodies -
Description
Mouse polyclonal to SMC3 -
Host species
Mouse -
Tested Applications & Species
See all applications and species dataApplication Species ICC/IF HumanWB Human
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Immunogen
Full length human SMC3 protein (AAH47324).
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Positive control
- WB: human colon tissue lysate; SMC3 transfected 293T cell lysate ICC/IF: HeLa cell
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.4
Constituent: 2.68% PBS -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-SMC3 antibody (ab89618)Anti-SMC3 antibody (ab89618) at 1 µg/ml + human colon tissue lysate at 50 µg
Predicted band size: 142 kDa
Observed band size: 142 kDa
Additional bands at: 46 kDa, 50 kDa, 65 kDa. We are unsure as to the identity of these extra bands.
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Western blot - Anti-SMC3 antibody (ab89618)All lanes : Anti-SMC3 antibody (ab89618) at 1 µg/ml
Lane 1 : SMC3 transfected 293T cell lysate
Lane 2 : non transfected 293T cell lysate
Lysates/proteins at 25 µg per lane.
Predicted band size: 142 kDa
Observed band size: ~142 kDa why is the actual band size different from the predicted?
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Immunocytochemistry/ Immunofluorescence - Anti-SMC3 antibody (ab89618)Immunofluorescence analysis of SMC3 in HeLa cell labelled with ab89618 at 10 µg/ml
