Anti-SMC3 antibody (ab9263)
Key features and details
- Rabbit polyclonal to SMC3
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-SMC3 antibody
See all SMC3 primary antibodies -
Description
Rabbit polyclonal to SMC3 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species ICC/IF HumanIHC-P HumanWB Human
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Immunogen
Synthetic peptide corresponding to Human SMC3 conjugated to keyhole limpet haemocyanin. Which represents a portion within the last 100 amino acids GenBank PID 4885399.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7
Preservative: 0.09% Sodium azide
Constituents: 0.021% PBS, 1.764% Sodium citrate, 1.815% Tris -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
Antibodies were affinity purified using the peptide immobilized on solid support. Antibody concentration was determined by extinction coefficient: absorbance at 280 nmof 1.4 equals 1.0 mg of IgG. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Immunocytochemistry/ Immunofluorescence - Anti-SMC3 antibody (ab9263) Fukuda et al PLoS Genet. 2012 Feb;8(2):e1002485. doi: 10.1371/journal.pgen.1002485. Epub 2012 Feb 9. Fig S3. Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/
SMC3 is phosphorylated during prophase I.
(Part of panel B) Nuclear spreads of spermatocytes were labeled with anti-pS1083, anti-SMC3 (ab9263) and anti-SYCP3 antibodies. Arrowheads indicate the XY bivalent. Bars, 10 µm.
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Western blot - Anti-SMC3 antibody (ab9263)All lanes : Anti-SMC3 antibody (ab9263) at 1 µg/ml
Lane 1 : Human placenta tissue lysate - total protein (ab29745)
Lane 2 : K562 (Human erythromyeloblastoid leukemia cell line) Whole Cell Lysate
Lane 3 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Predicted band size: 141.5 kDa
Observed band size: 145 kDa why is the actual band size different from the predicted?
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Immunocytochemistry/ Immunofluorescence - Anti-SMC3 antibody (ab9263) This image is courtesy of Kirk McManus, University of British ColumbiaInterphase HeLa (Human epithelial cell line from cervix adenocarcinoma) cells stained with ab9263 (1/500).
The antibody gave predominantly nuclear staining in all interphase cells investigated.
ab9263 staining is shown in green. The cells were counterstained with DAPI (red).
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Immunocytochemistry/ Immunofluorescence - Anti-SMC3 antibody (ab9263)SMC3 signals (green) in a human spermatocyte at the zygotene-pachytene transition stage.
Wider signals correspond to synapsed areas, whereas narrower signals show non-synapsed regions.
This image was kindly supplied as part of the review submitted by Maria Oliver-Bonet.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SMC3 antibody (ab9263)ab9263 (4µg/ml) staining SMC3 in human colon using an automated system (DAKO Autostainer Plus). Using this protocol there is strong nuclear staining.
Sections were rehydrated and antigen retrieved with the 3-in-1 AR buffer citrate pH 6.0. Slides were blocked with peroxidase in 3% H2O2 in methanol for 10 minutes. They were then blocked with a protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 minutes and detected with an amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with hematoxylin and coverslipped under DePeX.Please note that, for manual staining, optimization of primary antibody concentration and incubation time is recommended. Signal amplification may be required.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SMC3 antibody (ab9263)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human testicular seminoma tissue labeling SMC3 with ab9263 at 1/1000 ( 1µg/ml). Detection: DAB.
