Anti-CD98 antibody [MEM-108] - Plasma Membrane Marker (ab2528)
![Anti-CD98 antibody [MEM-108] - Plasma Membrane Marker (ab2528)](https://www.abcam.com/ps/products/2/ab2528/Images/ab2528-454755-AntiCD98-antibody-Flow-cytometry-blood.jpg "Anti-CD98 antibody [MEM-108] - Plasma Membrane Marker (ab2528)")
Key features and details
- Mouse monoclonal [MEM-108] to CD98 - Plasma Membrane Marker
- Suitable for: Flow Cyt
- Reacts with: Human
- Isotype: IgG1
Overview
-
Product name
Anti-CD98 antibody [MEM-108] - Plasma Membrane Marker
See all CD98 primary antibodies -
Description
Mouse monoclonal [MEM-108] to CD98 - Plasma Membrane Marker -
Host species
Mouse -
Tested applications
Suitable for: Flow Cytmore details -
Species reactivity
Reacts with: Human -
Immunogen
Tissue, cells or virus corresponding to Human CD98. Burkitt’s lymphoma cell line Raji
-
Positive control
- Flow Cyt: Human peripheral blood monocytes.
-
General notes
CD98 (4F2) is involved in normal and neoplastic cell growth.
This product was changed from ascites to tissue culture supernatant on 24th January 2018. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.097% Sodium azide
Constituent: PBS -
Concentration information loading... -
Purity
Protein A purified -
Purification notes
This product has been purified from tissue culture supernatant. Purity >95% by SDS-PAGE. -
Primary antibody notes
CD98 (4F2) is involved in normal and neoplastic cell growth. -
Clonality
Monoclonal -
Clone number
MEM-108 -
Isotype
IgG1 -
Research areas
Images
-
Flow Cytometry - Anti-CD98 antibody [MEM-108] - Plasma Membrane Marker (ab2528)
Flow Cytometry analysis of human peripheral blood monocytes labelling CD98 with ab2528 at 4 μg/ml.
-
![Immunocytochemistry/ Immunofluorescence - Anti-CD98 antibody [MEM-108] - Plasma Membrane Marker (ab2528)](https://www.abcam.com/ps/products/2/ab2528/Images/ab2528-196056-anti-cd98-antibody-mem-108-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-CD98 antibody [MEM-108] - Plasma Membrane Marker (ab2528)ICC/IF image of ab2528 stained MCF7 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab2528, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96879, DyLight® 488 goat anti-mouse IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
-
![Western blot - Anti-CD98 antibody [MEM-108] - Plasma Membrane Marker (ab2528)](https://www.abcam.com/ps/products/2/ab2528/Images/ab2528-5915-anti-cd98-antibody-mem-108-western-blot.jpg)
Western blot - Anti-CD98 antibody [MEM-108] - Plasma Membrane Marker (ab2528)All lanes : Anti-CD98 antibody [MEM-108] - Plasma Membrane Marker (ab2528) at 5 µg/ml
Lane 1 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
Lane 2 : MCF7 (Human breast adenocarcinoma cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat polyclonal to Mouse IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilutionThe CD98 protein has a predicted molecular weight of 57 kDa, however it is extensively glycosylated. This antibody recognises a bands at 98-150 kDa, similar to what has been observed with other antibodies directed against this protein.
Abcam welcomes customer feedback and would appreciate any comments regarding this product and the data presented above.
