Anti-p53 (phospho T55) antibody [EPR17729] - BSA and Azide free (ab250672)
![Anti-p53 (phospho T55) antibody [EPR17729] - BSA and Azide free (ab250672)](https://www.abcam.com/ps/products/250/ab250672/Images/ab250672-407940-anti-p53-phospho-t55-antibody-epr17729-bsa-and-azide-free-western-blot.jpg "Anti-p53 (phospho T55) antibody [EPR17729] - BSA and Azide free (ab250672)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR17729] to p53 (phospho T55) - BSA and Azide free
- Suitable for: Dot blot, WB
- Reacts with: Human
Overview
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Product name
Anti-p53 (phospho T55) antibody [EPR17729] - BSA and Azide free
See all p53 primary antibodies -
Description
Rabbit monoclonal [EPR17729] to p53 (phospho T55) - BSA and Azide free -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB Human
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Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: Whole cell lysate of HEK-293 cells treated with 200nM Calyculin A, 60J/m2 UV and/or 1uM Okadaic Acid for 60 minutes.
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General notes
Ab250672 is the carrier-free version of ab183546. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
ab250672 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR17729 -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-p53 (phospho T55) antibody [EPR17729] - BSA and Azide free (ab250672)All lanes : Anti-p53 (phospho T55) antibody [EPR17729] (ab183546) at 1/1000 dilution
Lane 1 : HEK-293 (Human epithelial cell line from embryonic kidney) whole cell lysate
Lane 2 : HEK-293 (Human epithelial cell line from embryonic kidney) whole cell lysate membrane incubated with phosphatase
Lane 3 : Whole cell lysate from HEK-293 (Human epithelial cell line from embryonic kidney) cells, treated with UV at 60J/m2 for 2 hours
Lysates/proteins at 15 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 43 kDa
Observed band size: 53 kDa why is the actual band size different from the predicted?
Exposure time: 10 secondsThis data was developed using ab183546, the same antibody clone in a different buffer formulation.
Blocking and dilution buffer: 5% NFDM/TBST.
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![Western blot - Anti-p53 (phospho T55) antibody [EPR17729] - BSA and Azide free (ab250672)](https://www.abcam.com/ps/products/250/ab250672/Images/ab250672-406043-anti-p53-phospho-t55-antibody-epr17729-bsa-and-azide-free-western-blot.jpg)
Western blot - Anti-p53 (phospho T55) antibody [EPR17729] - BSA and Azide free (ab250672)All lanes : Anti-p53 (phospho T55) antibody [EPR17729] (ab183546) at 1/1000 dilution
Lane 1 : HEK-293 (Human epithelial cells from embryonic kidney) whole cell lysate treated with 200nM Calyculin A and 1uM Okadaic Acid for 60 minutes.
Lane 2 : Untreated HEK293 (human embryonic kidney) whole cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution
Predicted band size: 43 kDa
Observed band size: 53 kDa why is the actual band size different from the predicted?This data was developed using ab183546, the same antibody clone in a different buffer formulation.
Blocking and dilution buffer: 5% NFDM/TBST.
Exposure time: 15 seconds.
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![Dot Blot - Anti-p53 (phospho T55) antibody [EPR17729] - BSA and Azide free (ab250672)](https://www.abcam.com/ps/products/250/ab250672/Images/ab250672-1-anti-p53-phospho-t55-antibody-epr17729-bsa-and-azide-free-dot-blot.jpg)
Dot Blot - Anti-p53 (phospho T55) antibody [EPR17729] - BSA and Azide free (ab250672)This data was developed using ab183546, the same antibody clone in a different buffer formulation.Dot blot analysis of p53 (phospho T55) using ab183546 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated secondary antibody at 1/1000 dilution.Lane 1: Phospho peptideLane 2: Non-phospho peptideBlocking/Dilution buffer: 5% NFDM/TBST.Exposure time of 3 minutes. -
![Anti-p53 (phospho T55) antibody [EPR17729] - BSA and Azide free (ab250672)](https://www.abcam.com/ps/products/250/ab250672/Images/ab250672-5-benefits-of-recombinant-antibodies.png)
Anti-p53 (phospho T55) antibody [EPR17729] - BSA and Azide free (ab250672)