Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-CEACAM6 antibody [EPR23956-80] - BSA and Azide free (ab275033)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR23956-80] to CEACAM6 - BSA and Azide free
  • Suitable for: IHC-P, Flow Cyt, ICC, WB, IP
  • Reacts with: Human

Overview

  • Product name

    Anti-CEACAM6 antibody [EPR23956-80] - BSA and Azide free
    See all CEACAM6 primary antibodies

  • Description

    Rabbit monoclonal [EPR23956-80] to CEACAM6 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: IHC-P, Flow Cyt, ICC, WB, IPmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: A549, BxPC-3 and LoVo whole cell lysates; Human lung tissue lysate; His-tagged human CEACAM6 recombinant protein. IHC-P: Human spleen and colon cancer tissue. ICC: BxPC-3 cells. Flow cyt: BxPC-3 cells.

  • General notes

    ab275033 is the carrier-free version of ab275022. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab275033 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C. Do Not Freeze.

  • Storage buffer

    Constituent: PBS

  • Carrier free

    Yes
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR23956-80

  • Isotype

    IgG

  • Research areas

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CEACAM6 antibody [EPR23956-80] - BSA and Azide free (ab275033)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CEACAM6 antibody [EPR23956-80] - BSA and Azide free (ab275033)

    This data was developed using ab275022, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue labeling CEACAM6 with abab275022 at 1/2000 (0.262 ug/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on human colon cancer (PMID: 27284373). The section was incubated with ab275022 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

  • Immunocytochemistry - Anti-CEACAM6 antibody [EPR23956-80] - BSA and Azide free (ab275033)
    Immunocytochemistry - Anti-CEACAM6 antibody [EPR23956-80] - BSA and Azide free (ab275033)

    This data was developed using ab275022, the same antibody clone in a different buffer formulation.

    Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized BxPC-3 (human pancreas adenocarcinoma cell) cells labelling CEACAM6 with ab275022 at 1/500 (1.048 ug/ml) dilution, followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution (Green). Confocal image showing membranous and cytoplamic staining in BxPC-3 cell line. Low expression control: LNCaP: PMID: 16204051. ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 dilution (Red). The counterstain was DAPI (Blue).

    Secondary antibody only control: Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution.

  • Immunoprecipitation - Anti-CEACAM6 antibody [EPR23956-80] - BSA and Azide free (ab275033)
    Immunoprecipitation - Anti-CEACAM6 antibody [EPR23956-80] - BSA and Azide free (ab275033)

    This data was developed using ab275022, the same antibody clone in a different buffer formulation.

    CEACAM6 was immunoprecipitated from 0.35 mg A549 (human lung carcinoma epithelial cell) whole cell lysate with ab275022 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab275022 at 1/2000 dilution. VeriBlot for IP Detection Reagent (HRP)(ab131366) was used at 1/5000 dilution.

    Lane 1: A549 (human lung carcinoma epithelial cell) whole cell lysate 10 ug

    Lane 2: ab275022 IP in A549 whole cell lysate

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab275022 in A549 whole cell lysate

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 3 seconds.

     

  • Flow Cytometry - Anti-CEACAM6 antibody [EPR23956-80] - BSA and Azide free (ab275033)
    Flow Cytometry - Anti-CEACAM6 antibody [EPR23956-80] - BSA and Azide free (ab275033)

    This data was developed using ab275022, the same antibody clone in a different buffer formulation.

    Flow cytometric analysis of LNCaP (human prostate carcinoma epithelial cell, Left) / BxPC-3 (human pancreas adenocarcinoma epithelial cell, Right) cells labelling CEACAM6 with ab275022 at 1/500 dilution (0.1ug) (Red) compared with a Rabbit monoclonal IgG (ab172730) isotype control (Black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).

    Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody.

    Negative control: LNCaP.

    Gated on viable cells.

  • Western blot - Anti-CEACAM6 antibody [EPR23956-80] - BSA and Azide free (ab275033)
    Western blot - Anti-CEACAM6 antibody [EPR23956-80] - BSA and Azide free (ab275033)
    All lanes : Anti-CEACAM6 antibody [EPR23956-80] (ab275022) at 1/1000 dilution

    Lane 1 : His-tagged human CEACAM6 recombinant protein, 20ng
    Lane 2 : His-tagged human CEACAM1 recombinant protein (aa35-428), 20ng
    Lane 3 : His-tagged human CEACAM5 recombinant protein (aa35-685), 20ng

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/100000 dilution

    Predicted band size: 37 kDa



    This data was developed using ab275022, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

    Exposure time: 15 seconds.

    The other family members have lower homology than the tested proteins.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CEACAM6 antibody [EPR23956-80] - BSA and Azide free (ab275033)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CEACAM6 antibody [EPR23956-80] - BSA and Azide free (ab275033)

    This data was developed using ab275022, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded human spleen tissue labeling CEACAM6 with abab275022 at 1/2000 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on human spleen. The section was incubated with ab275022 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

  • Western blot - Anti-CEACAM6 antibody [EPR23956-80] - BSA and Azide free (ab275033)
    Western blot - Anti-CEACAM6 antibody [EPR23956-80] - BSA and Azide free (ab275033)
    Anti-CEACAM6 antibody [EPR23956-80] (ab275022) at 1/1000 dilution + Human lung tissue lysate at 20 µg

    Secondary
    VeriBlot for IP secondary antibody(HRP)(ab131366) at 1/1000 dilution

    Predicted band size: 37 kDa
    Observed band size: 40-100 kDa why is the actual band size different from the predicted?



    This data was developed using ab275022, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

    Exposure time: 26 seconds.

    The molecular weight observed is consistent with what has been described in the literature (PMID: 19244123, 28892050, 16204051).

  • Western blot - Anti-CEACAM6 antibody [EPR23956-80] - BSA and Azide free (ab275033)
    Western blot - Anti-CEACAM6 antibody [EPR23956-80] - BSA and Azide free (ab275033)
    All lanes : Anti-CEACAM6 antibody [EPR23956-80] (ab275022) at 1/1000 dilution

    Lane 2 : LNCaP (human prostate carcinoma epithelial cell) whole cell lysate
    Lane 3 : BxPC-3 (human pancreas adenocarcinoma epithelial cell) whole cell lysate
    Lane 4 : LoVo (human colorectal adenocarcinoma epithelial cell) whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/100000 dilution

    Predicted band size: 37 kDa
    Observed band size: 40-100 kDa why is the actual band size different from the predicted?



    This data was developed using ab275022, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

    Exposure times: Lanes 1-2: 15 seconds; Lane3: 3 seconds; Lane 4: 26 seconds.

    The molecular weight observed is consistent with what has been described in the literature (PMID: 19244123, 28892050, 16204051).

    Low expression control: LNCaP (PMID: 16204051).

  • Immunoprecipitation - Anti-CEACAM6 antibody [EPR23956-80] - BSA and Azide free (ab275033)
    Immunoprecipitation - Anti-CEACAM6 antibody [EPR23956-80] - BSA and Azide free (ab275033)

    This data was developed using ab275022, the same antibody clone in a different buffer formulation.

    CEACAM6 was immunoprecipitated from 0.35 mg BxPC-3 (human pancreas adenocarcinoma epithelial cell) whole cell lysate with ab275022 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab275022 at 1/2000 dilution. VeriBlot for IP Detection Reagent (HRP)(ab131366) was used at 1/5000 dilution.

    Lane 1: BxPC-3 (human pancreas adenocarcinoma epithelial cell) whole cell lysate 10 ug

    Lane 2: ab275022 IP in BxPC-3 whole cell lysate

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab275022 in BxPC-3 whole cell lysate

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 3 seconds.

     

  • Anti-CEACAM6 antibody [EPR23956-80] - BSA and Azide free (ab275033)
    Anti-CEACAM6 antibody [EPR23956-80] - BSA and Azide free (ab275033)

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