Antibodies, Proteins, Kits and Reagents for Life Science

328 339 ₸ Product size

100 µl 328 339 ₸ 10 µl 134 016 ₸

Order now and get it on Tuesday March 02, 2021

Anti-CD98 antibody [EPR3548(2)] (ab108300)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR3548(2)] to CD98
Suitable for: WB, IP, IHC-P
Reacts with: Human

Product name

Anti-CD98 antibody [EPR3548(2)]
See all CD98 primary antibodies
Description

Rabbit monoclonal [EPR3548(2)] to CD98
Host species

Rabbit
Specificity

Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Tested Applications & Species

Application Species
IHC-P
Human

WB
Human

See all applications and species data
Immunogen

A synthetic peptide corresponding to residues within the extracellular region of Human CD98
Positive control
- WB: HeLa, HepG2, Raji, and Molt-4 cell lysates; IHC-P: Human brain and Human testis tissue.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Application	Species
IHC-P	Human
WB	Human

Form

Liquid
Storage instructions

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer

pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
Concentration information loading...
Purity

Tissue culture supernatant
Clonality

Monoclonal
Clone number

EPR3548(2)
Isotype

IgG
Research areas

Western blot - Anti-CD98 antibody [EPR3548(2)] (ab108300)

All lanes : Anti-CD98 antibody [EPR3548(2)] (ab108300) at 1/1000 dilution

Lane 1 : HeLa cell lysate
Lane 2 : HepG2 cell lysate
Lane 3 : Raji cell lysate
Lane 4 : Molt-4 cell lysate

Lysates/proteins at 10 µg per lane.

Predicted band size: 68 kDa
Observed band size: 80 kDa
why is the actual band size different from the predicted?
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD98 antibody [EPR3548(2)] (ab108300)

Immunohistochemical analysis of paraffin-embedded Human brain tissue using ab108300 at a dilution of 1/100.

Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD98 antibody [EPR3548(2)] (ab108300)

Immunohistochemical analysis of paraffin-embedded Human testis tissue using ab108300 at a dilution of 1/100.

Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.
Western blot - Anti-CD98 antibody [EPR3548(2)] (ab108300)

All lanes : Anti-CD98 antibody [EPR3548(2)] (ab108300) at 1/1000 dilution

Lane 1 : HeLa (Human cervix adenocarcinoma epithelial cell), whole cell lysate prepared in RIPA lysis method
Lane 2 : HeLa (Human cervix adenocarcinoma epithelial cell), whole cell lysate prepared in 1% SDS Hot lysis method

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

Predicted band size: 68 kDa
Observed band size: 80 kDa why is the actual band size different from the predicted?

Exposure time: 180 seconds

Blocking/Diluting buffer and concentration: 5% NFDM/TBST.

Rabbit monoclonal [EPR16891] to GAPDH (ab181602) used as loading control.
Anti-CD98 antibody [EPR3548(2)] (ab108300)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com