Antibodies, Proteins, Kits and Reagents for Life Science

351 792 ₸ Product size

100 µl 351 792 ₸ 10 µl 140 716 ₸

Order now and get it on Tuesday March 02, 2021

Anti-Cytokeratin 13 antibody [EPR3671] (ab92551)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR3671] to Cytokeratin 13
Suitable for: WB, ICC, IHC-P, Flow Cyt
Reacts with: Human

Product name

Anti-Cytokeratin 13 antibody [EPR3671]
See all Cytokeratin 13 primary antibodies
Description

Rabbit monoclonal [EPR3671] to Cytokeratin 13
Host species

Rabbit

Tested Applications & Species

Application	Species
Flow Cyt	Human
ICC	Human
IHC-P	Human
WB	Human

See all applications and species data

Immunogen

Synthetic peptide within Human Cytokeratin 13 aa 100-200. The exact sequence is proprietary.
(Peptide available as ab180923)
Positive control
- WB: A431, HACAT or 293T cell lysate IHC: transitional cell urinary bladder carcinoma tissue Flow Cyt: A431 cells ICC: A431 cells
General notes
Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
Dissociation constant (K_D)

K_D = 1.20 x 10 ^-11 M
Learn more about K_D
Storage buffer

pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.5% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR3671
Isotype

IgG
Research areas

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 13 antibody [EPR3671] (ab92551)

ab92551 at 1/100 dilution staining Cytokeratin 13 in formalin-fixed, paraffin-embedded human transitional cell usrinary bladder carcinoma tissue by immunohistochemistry. Detection: DAB staining. Antigen retrieval was heat mediated via the pressure cooker method before commencing with IHC staining protocol.
Western blot - Anti-Cytokeratin 13 antibody [EPR3671] (ab92551)

All lanes : Anti-Cytokeratin 13 antibody [EPR3671] (ab92551) at 1/100000 dilution

Lane 1 : A431 cell lysate
Lane 2 : HACAT cell lysate
Lane 3 : 293T cell lysate

Lysates/proteins at 10 µg per lane.

Secondary
Lanes 1-2 : HRP labelled Goat anti-Rabbit IgG at 1/2000 dilution
Lane 3 : HRP labelled goat anti-Rabbit IgG at 1/2000 dilution

Predicted band size: 50 kDa
Immunocytochemistry - Anti-Cytokeratin 13 antibody [EPR3671] (ab92551)

Immunocytochemistry analysis of A431 (human epidermoid carcinoma epithelial cell) cells labeling Cytokeratin 13 with ab92551 at 1/500 (4 μg/mL). Cells were fixed with 4% Paraformaldehyde and permeabilised with 0.1% tritonX-100. ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor^® 594) at 1/200 (2.5 μg/mL) was used to counterstain the cells. ab150077 AlexaFluor^®488 Goat anti-Rabbit at 1/1000 (2 μg/mL) was used as the secondary antibody. DAPI (blue) was used as nuclear counterstain.

Confocal image showing cytoplasmic staining in A431 cells.
Flow Cytometry - Anti-Cytokeratin 13 antibody [EPR3671] (ab92551)

Flow Cytometry analysis of A431 (human epidermoid carcinoma) cells labeling Cytokeratin 13 with purified ab92551 at 1/20 dilution (red). The secondary antibody was Goat anti rabbit IgG (Alexa Fluor^® 488) at 1/2000 dilution. A Rabbit monoclonal IgG (Black) was used as the isotype control and cells without incubation with primary antibody and secondary antibody (Blue) were used as unlabeled control.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 13 antibody [EPR3671] (ab92551)

ab92551 showing positive staining in Cervical human carcinoma tissue. Antigen retrieval was heat mediated via the pressure cooker method before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 13 antibody [EPR3671] (ab92551)

ab92551 showing positive staining in Normal human tonsil squamous cells tissue. Antigen retrieval was heat mediated via the pressure cooker method before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 13 antibody [EPR3671] (ab92551)

ab92551 showing negative staining in human Glioma tissue. Antigen retrieval was heat mediated via the pressure cooker method before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 13 antibody [EPR3671] (ab92551)

ab92551 showing negative staining in human Breast carcinoma tissue. Antigen retrieval was heat mediated via the pressure cooker method before commencing with IHC staining protocol.
OI-RD Scanning - Anti-Cytokeratin 13 antibody [EPR3671] (ab92551)

Equilibrium disassociation constant (K_D)
Learn more about K_D

Click here to learn more about K_D
Anti-Cytokeratin 13 antibody [EPR3671] (ab92551)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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