Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Cytokeratin 13 antibody [EPR3671] (ab92551)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR3671] to Cytokeratin 13
  • Suitable for: WB, ICC, IHC-P, Flow Cyt
  • Reacts with: Human

Overview

  • Product name

    Anti-Cytokeratin 13 antibody [EPR3671]
    See all Cytokeratin 13 primary antibodies

  • Description

    Rabbit monoclonal [EPR3671] to Cytokeratin 13

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    ICC
    Human
    IHC-P
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide within Human Cytokeratin 13 aa 100-200. The exact sequence is proprietary.
    (Peptide available as ab180923)

  • Positive control

    • WB: A431, HACAT or 293T cell lysate IHC: transitional cell urinary bladder carcinoma tissue Flow Cyt: A431 cells ICC: A431 cells

  • General notes

     

    Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 13 antibody [EPR3671] (ab92551)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 13 antibody [EPR3671] (ab92551)

    ab92551 at 1/100 dilution staining Cytokeratin 13 in formalin-fixed, paraffin-embedded human transitional cell usrinary bladder carcinoma tissue by immunohistochemistry. Detection: DAB staining. Antigen retrieval was heat mediated via the pressure cooker method before commencing with IHC staining protocol.

  • Western blot - Anti-Cytokeratin 13 antibody [EPR3671] (ab92551)
    Western blot - Anti-Cytokeratin 13 antibody [EPR3671] (ab92551)
    All lanes : Anti-Cytokeratin 13 antibody [EPR3671] (ab92551) at 1/100000 dilution

    Lane 1 : A431 cell lysate
    Lane 2 : HACAT cell lysate
    Lane 3 : 293T cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Lanes 1-2 : HRP labelled Goat anti-Rabbit IgG at 1/2000 dilution
    Lane 3 : HRP labelled goat anti-Rabbit IgG at 1/2000 dilution

    Predicted band size: 50 kDa

  • Immunocytochemistry - Anti-Cytokeratin 13 antibody [EPR3671] (ab92551)
    Immunocytochemistry - Anti-Cytokeratin 13 antibody [EPR3671] (ab92551)

    Immunocytochemistry analysis of A431 (human epidermoid carcinoma epithelial cell) cells labeling Cytokeratin 13 with ab92551 at 1/500 (4 μg/mL). Cells were fixed with 4% Paraformaldehyde and permeabilised with 0.1% tritonX-100. ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) at 1/200 (2.5 μg/mL) was used to counterstain the cells. ab150077 AlexaFluor®488 Goat anti-Rabbit at 1/1000 (2 μg/mL) was used as the secondary antibody. DAPI (blue) was used as nuclear counterstain.

    Confocal image showing cytoplasmic staining in A431 cells.

     

  • Flow Cytometry - Anti-Cytokeratin 13 antibody [EPR3671] (ab92551)
    Flow Cytometry - Anti-Cytokeratin 13 antibody [EPR3671] (ab92551)

    Flow Cytometry analysis of A431 (human epidermoid carcinoma) cells labeling Cytokeratin 13 with purified ab92551 at 1/20 dilution (red). The secondary antibody was Goat anti rabbit IgG (Alexa Fluor® 488) at 1/2000 dilution. A Rabbit monoclonal IgG (Black) was used as the isotype control and cells without incubation with primary antibody and secondary antibody (Blue) were used as unlabeled control.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 13 antibody [EPR3671] (ab92551)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 13 antibody [EPR3671] (ab92551)

    ab92551 showing positive staining in Cervical human carcinoma tissue. Antigen retrieval was heat mediated via the pressure cooker method before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 13 antibody [EPR3671] (ab92551)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 13 antibody [EPR3671] (ab92551)

    ab92551 showing positive staining in Normal human tonsil squamous cells tissue. Antigen retrieval was heat mediated via the pressure cooker method before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 13 antibody [EPR3671] (ab92551)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 13 antibody [EPR3671] (ab92551)

    ab92551 showing negative staining in human Glioma tissue. Antigen retrieval was heat mediated via the pressure cooker method before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 13 antibody [EPR3671] (ab92551)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 13 antibody [EPR3671] (ab92551)

    ab92551 showing negative staining in human Breast carcinoma tissue. Antigen retrieval was heat mediated via the pressure cooker method before commencing with IHC staining protocol.

  • OI-RD Scanning - Anti-Cytokeratin 13 antibody [EPR3671] (ab92551)
    OI-RD Scanning - Anti-Cytokeratin 13 antibody [EPR3671] (ab92551)
    Equilibrium disassociation constant (KD)
    Learn more about KD

    Click here to learn more about KD
  • Anti-Cytokeratin 13 antibody [EPR3671] (ab92551)
    Anti-Cytokeratin 13 antibody [EPR3671] (ab92551)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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