Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-RUNX1 / AML1+RUNX3+RUNX2 antibody [EPR3098] - BSA and Azide free (ab248432)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR3098] to RUNX1 / AML1+RUNX3+RUNX2 - BSA and Azide free
  • Suitable for: WB, IHC-P
  • Reacts with: Mouse, Human

Overview

  • Product name

    Anti-RUNX1 / AML1+RUNX3+RUNX2 antibody [EPR3098] - BSA and Azide free
    See all RUNX1 / AML1+RUNX3+RUNX2 primary antibodies

  • Description

    Rabbit monoclonal [EPR3098] to RUNX1 / AML1+RUNX3+RUNX2 - BSA and Azide free

  • Host species

    Rabbit

  • Specificity

    The immunogen of this antibody is 100% homologous to mouse RUNX2 sequence.

  • Tested applications

    Suitable for: WB, IHC-Pmore details
    Unsuitable for: Flow Cyt,ICC/IF or IP

  • Species reactivity

    Reacts with: Mouse, Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    ab248432 is the carrier-free version of ab133261 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    Ab248432 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Rat: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Images

  • Western blot - Anti-RUNX1 / AML1+RUNX3+RUNX2 antibody [EPR3098] - BSA and Azide free (ab248432)
    Western blot - Anti-RUNX1 / AML1+RUNX3+RUNX2 antibody [EPR3098] - BSA and Azide free (ab248432)

    This data was developed using ab133261, the same antibody clone in a different buffer formulation.

    RUNX2 recombinant protein full length, with N-terminal HIS tag, expressed in E.Coli.

    RUNX3 overexpression and empty vector control lysates created in HEK293T cells. The protein contains a C-terminal DDK tag.

  • Western blot - Anti-RUNX1 / AML1+RUNX3+RUNX2 antibody [EPR3098] - BSA and Azide free (ab248432)
    Western blot - Anti-RUNX1 / AML1+RUNX3+RUNX2 antibody [EPR3098] - BSA and Azide free (ab248432)
    All lanes : Anti-RUNX1 / AML1 + RUNX3 + RUNX2 antibody [EPR3098] (ab133261) at 1/1000 dilution

    Lane 1 : Molt 4 cell lysate
    Lane 2 : Human fetal thymus lysate
    Lane 3 : Mouse thymus lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat anti-rabbit HRP conjugated antibody at 1/2000 dilution

    Predicted band size: 48 kDa



    This data was developed using ab133261, the same antibody clone in a different buffer formulation.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RUNX1 / AML1+RUNX3+RUNX2 antibody [EPR3098] - BSA and Azide free (ab248432)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RUNX1 / AML1+RUNX3+RUNX2 antibody [EPR3098] - BSA and Azide free (ab248432)

    This data was developed using ab133261, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin embedded Human tonsil tissue labelling RUNX1 / AML1 with ab133261 at 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RUNX1 / AML1+RUNX3+RUNX2 antibody [EPR3098] - BSA and Azide free (ab248432)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RUNX1 / AML1+RUNX3+RUNX2 antibody [EPR3098] - BSA and Azide free (ab248432)
    This data was developed using ab133261, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin embedded normal Human spleen tissue using ab133261 showing +ve staining.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RUNX1 / AML1+RUNX3+RUNX2 antibody [EPR3098] - BSA and Azide free (ab248432)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RUNX1 / AML1+RUNX3+RUNX2 antibody [EPR3098] - BSA and Azide free (ab248432)
    This data was developed using ab133261, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin embedded normal Human testis tissue using ab133261 showing +ve staining.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RUNX1 / AML1+RUNX3+RUNX2 antibody [EPR3098] - BSA and Azide free (ab248432)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RUNX1 / AML1+RUNX3+RUNX2 antibody [EPR3098] - BSA and Azide free (ab248432)
    This data was developed using ab133261, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin embedded normal Human heart tissue using ab133261 showing -ve staining.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RUNX1 / AML1+RUNX3+RUNX2 antibody [EPR3098] - BSA and Azide free (ab248432)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RUNX1 / AML1+RUNX3+RUNX2 antibody [EPR3098] - BSA and Azide free (ab248432)
    This data was developed using ab133261, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin embedded normal Human colon tissue using ab133261 showing +ve staining.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RUNX1 / AML1+RUNX3+RUNX2 antibody [EPR3098] - BSA and Azide free (ab248432)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RUNX1 / AML1+RUNX3+RUNX2 antibody [EPR3098] - BSA and Azide free (ab248432)
    This data was developed using ab133261, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin embedded normal Human breast tissue using ab133261 showing +ve staining.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
  • Anti-RUNX1 / AML1+RUNX3+RUNX2 antibody [EPR3098] - BSA and Azide free (ab248432)
    Anti-RUNX1 / AML1+RUNX3+RUNX2 antibody [EPR3098] - BSA and Azide free (ab248432)

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