Antibodies, Proteins, Kits and Reagents for Life Science

307 566 ₸ Product size

100 µg 307 566 ₸ 10 µg 103 862 ₸

Order now and get it on Wednesday March 10, 2021

Anti-Endothelin A Receptor/ET-A antibody [UMB-8-37-1] (ab178454)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [UMB-8-37-1] to Endothelin A Receptor/ET-A
Suitable for: IHC-P, WB
Reacts with: Mouse, Rat, Human

Product name

Anti-Endothelin A Receptor/ET-A antibody [UMB-8-37-1]
See all Endothelin A Receptor/ET-A primary antibodies
Description

Rabbit monoclonal [UMB-8-37-1] to Endothelin A Receptor/ET-A
Host species

Rabbit

Tested Applications & Species

Application	Species
IHC-P	Mouse Rat Human
WB	Mouse Recombinant fragment

See all applications and species data

Immunogen

Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: Mouse heart and lung tissue lysates; IHC-P: Mouse and rat cardiac muscle and human placenta tissue.
General notes
This product has switched from a hybridoma to recombinant production method on 9^th September 2020.

The epitope recognized by the UMB-8-37-1 monoclonal contains phosphorylation sites that affect signal in Western blot after treatment with endothelin. Phosphatase treatment may be required prior to Western blot in order to improve signal (PubMed ID 25708416).

This antibody clone is manufactured by Abcam. If you require a different buffer formulation or a particular conjugate for your experiments, please contact orders@abcam.com.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.40
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Some batches contain 6.97% L-Arginine as a stabilizing agent. For lot-specific buffer information, please contact our Scientific Support team.
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

UMB-8-37-1
Isotype

IgG
Research areas

Western blot - Anti-Endothelin A Receptor/ET-A antibody [UMB-8-37-1] (ab178454)

All lanes : Anti-Endothelin A Receptor/ET-A antibody [UMB-8-37-1] (ab178454) at 1/1000 dilution

Lane 1 : Mouse heart lysate
Lane 2 : Mouse lung lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Developed using the ECL technique.

Performed under reducing conditions.

Predicted band size: 40-70 kDa
Observed band size: 40-70 kDa

Exposure time: 26 seconds

Blocking and diluting buffer and concentration: 5% NFDM/TBST.

The observed molecular weight is consistent with what has been described in the literature (PMID: 25708416).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Endothelin A Receptor/ET-A antibody [UMB-8-37-1] (ab178454)

Immunohistochemical analysis of paraffin-embedded human placenta tissue labeling Endothelin A Receptor/ET-A with ab178454 at 1/100 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Counterstained with hematoxylin. Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP). Perform heat mediated antigen retrieval using Tris/EDTA buffer (pH 9.0).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Endothelin A Receptor/ET-A antibody [UMB-8-37-1] (ab178454)

Immunohistochemical analysis of paraffin-embedded rat cardiac muscle tissue labeling Endothelin A Receptor/ET-A with ab178454 at 1/100 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Counterstained with hematoxylin. Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP). Perform heat mediated antigen retrieval using Tris/EDTA buffer (pH 9.0).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Endothelin A Receptor/ET-A antibody [UMB-8-37-1] (ab178454)

Immunohistochemical analysis of paraffin-embedded mouse cardiac muscle tissue labeling Endothelin A Receptor/ET-A with ab178454 at 1/100 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Counterstained with hematoxylin. Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP). Perform heat mediated antigen retrieval using Tris/EDTA buffer (pH 9.0).
Western blot - Anti-Endothelin A Receptor/ET-A antibody [UMB-8-37-1] (ab178454)

All lanes : Anti-Endothelin A Receptor/ET-A antibody [UMB-8-37-1] (ab178454) at 1/1000 dilution

Lane 1 : HEK-293 (human embryonic kidney) transfected with an empty vector (vector control), containing a myc-His-tag®, whole cell lysate
Lane 2 : HEK-293 transfected with Endothelin A Receptor/ET-A (WT) expression vector containing a myc-His-tag® , whole cell lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Developed using the ECL technique.

Performed under reducing conditions.

Predicted band size: 40-70 kDa
Observed band size: 40-80 kDa
why is the actual band size different from the predicted?

Exposure time: 3 minutes

Blocking and diluting buffer and concentration: 5% NFDM/TBST.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Endothelin A Receptor/ET-A antibody [UMB-8-37-1] (ab178454) Lupp A et al., Peptides, 66, 19-25, Fig 3,; doi: 10.1016/j.peptides.2015.02.005. With permission from Elsevier

Immunohistochemical localization of ETA in human normal lymph node (I) and in neoplastic tissues (A–H) using ab178454. For more protocol information please see PMID: 25708416.

Image generated from hybridoma version of ab178454
Anti-Endothelin A Receptor/ET-A antibody [UMB-8-37-1] (ab178454)

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