Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Endothelin A Receptor/ET-A antibody [UMB-8-37-1] (ab178454)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [UMB-8-37-1] to Endothelin A Receptor/ET-A
  • Suitable for: IHC-P, WB
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-Endothelin A Receptor/ET-A antibody [UMB-8-37-1]
    See all Endothelin A Receptor/ET-A primary antibodies

  • Description

    Rabbit monoclonal [UMB-8-37-1] to Endothelin A Receptor/ET-A

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    IHC-P
    Mouse
    Rat
    Human
    WB
    Mouse
    Recombinant fragment
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Mouse heart and lung tissue lysates; IHC-P: Mouse and rat cardiac muscle and human placenta tissue.

  • General notes

    This product has switched from a hybridoma to recombinant production method on 9th September 2020.

    The epitope recognized by the UMB-8-37-1 monoclonal contains phosphorylation sites that affect signal in Western blot after treatment with endothelin. Phosphatase treatment may be required prior to Western blot in order to improve signal (PubMed ID 25708416).

    This antibody clone is manufactured by Abcam. If you require a different buffer formulation or a particular conjugate for your experiments, please contact orders@abcam.com.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

Properties

Images

  • Western blot - Anti-Endothelin A Receptor/ET-A antibody [UMB-8-37-1] (ab178454)
    Western blot - Anti-Endothelin A Receptor/ET-A antibody [UMB-8-37-1] (ab178454)
    All lanes : Anti-Endothelin A Receptor/ET-A antibody [UMB-8-37-1] (ab178454) at 1/1000 dilution

    Lane 1 : Mouse heart lysate
    Lane 2 : Mouse lung lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 40-70 kDa
    Observed band size: 40-70 kDa


    Exposure time: 26 seconds


    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    The observed molecular weight is consistent with what has been described in the literature (PMID: 25708416).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Endothelin A Receptor/ET-A antibody [UMB-8-37-1] (ab178454)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Endothelin A Receptor/ET-A antibody [UMB-8-37-1] (ab178454)

    Immunohistochemical analysis of paraffin-embedded human placenta tissue labeling Endothelin A Receptor/ET-A with ab178454 at 1/100 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Counterstained with hematoxylin. Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP). Perform heat mediated antigen retrieval using Tris/EDTA buffer (pH 9.0).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Endothelin A Receptor/ET-A antibody [UMB-8-37-1] (ab178454)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Endothelin A Receptor/ET-A antibody [UMB-8-37-1] (ab178454)

    Immunohistochemical analysis of paraffin-embedded rat cardiac muscle tissue labeling Endothelin A Receptor/ET-A with ab178454 at 1/100 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Counterstained with hematoxylin. Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP). Perform heat mediated antigen retrieval using Tris/EDTA buffer (pH 9.0).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Endothelin A Receptor/ET-A antibody [UMB-8-37-1] (ab178454)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Endothelin A Receptor/ET-A antibody [UMB-8-37-1] (ab178454)

    Immunohistochemical analysis of paraffin-embedded mouse cardiac muscle tissue labeling Endothelin A Receptor/ET-A with ab178454 at 1/100 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Counterstained with hematoxylin. Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP). Perform heat mediated antigen retrieval using Tris/EDTA buffer (pH 9.0).

  • Western blot - Anti-Endothelin A Receptor/ET-A antibody [UMB-8-37-1] (ab178454)
    Western blot - Anti-Endothelin A Receptor/ET-A antibody [UMB-8-37-1] (ab178454)
    All lanes : Anti-Endothelin A Receptor/ET-A antibody [UMB-8-37-1] (ab178454) at 1/1000 dilution

    Lane 1 : HEK-293 (human embryonic kidney) transfected with an empty vector (vector control), containing a myc-His-tag®, whole cell lysate
    Lane 2 : HEK-293 transfected with Endothelin A Receptor/ET-A (WT) expression vector containing a myc-His-tag® , whole cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 40-70 kDa
    Observed band size: 40-80 kDa
    why is the actual band size different from the predicted?


    Exposure time: 3 minutes


    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Endothelin A Receptor/ET-A antibody [UMB-8-37-1] (ab178454)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Endothelin A Receptor/ET-A antibody [UMB-8-37-1] (ab178454) Lupp A et al., Peptides, 66, 19-25, Fig 3,; doi: 10.1016/j.peptides.2015.02.005. With permission from Elsevier

    Immunohistochemical localization of ETA in human normal lymph node (I) and in neoplastic tissues (A–H) using ab178454. For more protocol information please see PMID: 25708416.

    Image generated from hybridoma version of ab178454

  • Anti-Endothelin A Receptor/ET-A antibody [UMB-8-37-1] (ab178454)
    Anti-Endothelin A Receptor/ET-A antibody [UMB-8-37-1] (ab178454)

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