Anti-Endothelin A Receptor/ET-A antibody (ab85163)
Key features and details
- Rabbit polyclonal to Endothelin A Receptor/ET-A
- Suitable for: WB
- Reacts with: Rat
- Isotype: IgG
Overview
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Product name
Anti-Endothelin A Receptor/ET-A antibody
See all Endothelin A Receptor/ET-A primary antibodies -
Description
Rabbit polyclonal to Endothelin A Receptor/ET-A -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Rat
Predicted to work with: Mouse, Rabbit, Dog, Human
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Immunogen
Synthetic peptide corresponding to Rat Endothelin A Receptor/ET-A aa 100-200 conjugated to keyhole limpet haemocyanin.
(Peptide available asab93498) -
General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration
Concentration information loading...Purity
Immunogen affinity purifiedClonality
PolyclonalIsotype
IgGResearch areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab85163 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application Abreviews Notes WB Use a concentration of 1 µg/ml. Detects a band of approximately 46 kDa (predicted molecular weight: 48 kDa). Target
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Function
Receptor for endothelin-1. Mediates its action by association with G proteins that activate a phosphatidylinositol-calcium second messenger system. The rank order of binding affinities for ET-A is: ET1 > ET2 >> ET3. -
Tissue specificity
Isoform 1, isoform 3 and isoform 4 are expressed in a variety of tissues, with highest levels in the aorta and cerebellum, followed by lung, atrium and cerebral cortex, lower levels in the placenta, kidney, adrenal gland, duodenum, colon, ventricle and liver but no expression in umbilical vein endothelial cells. Within the placenta, isoform 1, isoform 2, isoform 3 and isoform 4 are expressed in the villi and stem villi vessels. -
Sequence similarities
Belongs to the G-protein coupled receptor 1 family. Endothelin receptor subfamily. EDNRA sub-subfamily. -
Cellular localization
Cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 450187 Dog
- Entrez Gene: 1909 Human
- Entrez Gene: 13617 Mouse
- Entrez Gene: 100126073 Rabbit
- Entrez Gene: 24326 Rat
- GenBank: NM_001957 Human
- GenBank: NM_174308 Human
- GenBank: NM_012550 Rat
see all -
Alternative names
- Ednra antibody
- EDNRA_HUMAN antibody
- Endothelin 1 receptor precursor antibody
see all
Images
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Western blot - Anti-Endothelin A Receptor/ET-A antibody (ab85163)All lanes : Anti-Endothelin A Receptor/ET-A antibody (ab85163) at 1 µg/ml
Lane 1 : Brain (Rat) Tissue Lysate
Lane 2 : Cerebellum Rat Tissue Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 48 kDa
Observed band size: 46 kDa why is the actual band size different from the predicted?
Exposure time: 20 minutes
The band observed at 46 kDa could potentially be a cleaved form of Rat Endothelin-1 receptor precursor, as the target protein contains a 20 amino acid signal peptide, which when removed gives an expected molecular weight of 46 kDa. -
Western blot - Anti-Endothelin A Receptor/ET-A antibody (ab85163)This image is courtesy of an Abreview submitted by Nico JentschAll lanes : Anti-Endothelin A Receptor/ET-A antibody (ab85163) at 1/250 dilution (in 5% BSA + TBS + 0.1% Tween20 for 12 hours at 4°C)
All lanes : Whole tissue lysate of rat lung
Lysates/proteins at 40 µg per lane.
Secondary
All lanes : An HRP-conjugated Goat anti-rabbit IgG polyclonal at 1/1000 dilution
Developed using the ECL technique.
Performed under non-reducing conditions.
Predicted band size: 48 kDa
Observed band size: 48 kDa
Exposure time: 30 seconds
Blocking step: 5% Milk for 1 hour at 23°C
Protocols
Datasheets and documents
References (9)
ab85163 has been referenced in 9 publications.
- Li L et al. Downregulation of endothelin A receptor (ETaR) ameliorates renal ischemia reperfusion injury by increasing nitric oxide production. Life Sci 228:295-304 (2019). PubMed: 31075232
- Silpanisong J et al. Chronic hypoxia alters fetal cerebrovascular responses to endothelin-1. Am J Physiol Cell Physiol 313:C207-C218 (2017). PubMed: 28566491
- Sun Y et al. Reduction of Uterine Perfusion Pressure Induced Redistribution of Endothelin Receptor Type-B Between the Intima and Media Contributes to the Pathogenesis of Pregnancy-Induced Hypertension. Cell Physiol Biochem 44:1715-1725 (2017). PubMed: 29216636
- Gulati S et al. Attenuation of opioid tolerance by ETB receptor agonist, IRL-1620, is independent of an accompanied decrease in nerve growth factor in mice. Heliyon 3:e00317 (2017). WB ; Mouse . PubMed: 28626808
- Yasir A et al. Diabetes-mediated middle cerebral artery remodeling is restored by linagliptin: Interaction with the vascular smooth muscle cell endothelin system. Life Sci 159:76-82 (2016). PubMed: 26944436
- Guh YJ et al. Endothelin-1 regulates H?-ATPase-dependent transepithelial H? secretion in zebrafish. Endocrinology 155:1728-37 (2014). PubMed: 24424055
- Díaz-García C et al. Pregnancy after allogeneic uterus transplantation in the rat: perinatal outcome and growth trajectory. Fertil Steril 102:1545-52.e1 (2014). PubMed: 25439799
- Joseph EK et al. Vascular endothelial cells mediate mechanical stimulation-induced enhancement of endothelin hyperalgesia via activation of P2X2/3 receptors on nociceptors. J Neurosci 33:2849-59 (2013). WB ; Rat . PubMed: 23407944
- Speed JS et al. Renal medullary endothelin-1 is decreased in Dahl salt-sensitive rats. Am J Physiol Regul Integr Comp Physiol 301:R519-23 (2011). WB ; Rat . PubMed: 21613578
Images
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Western blot - Anti-Endothelin A Receptor/ET-A antibody (ab85163)All lanes : Anti-Endothelin A Receptor/ET-A antibody (ab85163) at 1 µg/ml
Lane 1 : Brain (Rat) Tissue Lysate
Lane 2 : Cerebellum Rat Tissue Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 48 kDa
Observed band size: 46 kDa why is the actual band size different from the predicted?
Exposure time: 20 minutes
The band observed at 46 kDa could potentially be a cleaved form of Rat Endothelin-1 receptor precursor, as the target protein contains a 20 amino acid signal peptide, which when removed gives an expected molecular weight of 46 kDa. -
Western blot - Anti-Endothelin A Receptor/ET-A antibody (ab85163) This image is courtesy of an Abreview submitted by Nico JentschAll lanes : Anti-Endothelin A Receptor/ET-A antibody (ab85163) at 1/250 dilution (in 5% BSA + TBS + 0.1% Tween20 for 12 hours at 4°C)
All lanes : Whole tissue lysate of rat lung
Lysates/proteins at 40 µg per lane.
Secondary
All lanes : An HRP-conjugated Goat anti-rabbit IgG polyclonal at 1/1000 dilution
Developed using the ECL technique.
Performed under non-reducing conditions.
Predicted band size: 48 kDa
Observed band size: 48 kDa
Exposure time: 30 seconds
Blocking step: 5% Milk for 1 hour at 23°C
