Prostaglandin E2 ELISA Kit (ab133021)
Key features and details
- Sensitivity: 13.4 pg/ml
- Range: 39.1 pg/ml - 2500 pg/ml
- Sample type: Cell culture supernatant, Saliva, Serum, Urine, Whole Blood
- Detection method: Colorimetric
- Assay type: Competitive
- Reacts with: Species independent
Overview
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Product name
Prostaglandin E2 ELISA Kit
See all PGE2 kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Low PGE2 24 116pg/ml 8.9% Medium PGE2 24 492pg/ml 5.8% High PGE2 24 2416pg/ml 17.5% Inter-assay Sample n Mean SD CV% Low PGE2 8 111pg/ml 3% Medium PGE2 8 419pg/ml 5.1% High PGE2 8 1902pg/ml 3.9% -
Sample type
Cell culture supernatant, Saliva, Urine, Serum, Whole Blood -
Assay type
Competitive -
Sensitivity
13.4 pg/ml -
Range
39.1 pg/ml - 2500 pg/ml -
Recovery
Sample specific recovery Sample type Average % Range Saliva 123.3 % - % Urine 108.9 % - % Whole Blood 101.2 % - % Tissue Culture Media 104.4 % - % -
Assay time
3h 00m -
Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Species independent -
Product overview
PGE2 ELISA kit (Prostaglandin E2) is a competitive ELISA designed for the accurate quantitative measurement of Prostaglandin E2 in serum, saliva, urine and tissue culture media and other biological fluids.
A mouse IgG antibody has been precoated onto 96-well plates. Standards or test samples are added to the wells, along with an alkaline phosphatase (AP) conjugated-Prostaglandin E2 antibody. After incubation the excess reagents are washed away and pNpp substrate is added and is catalyzed by AP to produce a yellow color. The intensity of the yellow coloration is inversely proportional to the amount of Prostaglandin E2 captured in the plate.
This kit is unsuitable for mouse serum as samples containing mouse IgG may interfere with the assay.
Tissue/Cell lysate have not been internaly validated so we do not have specific protocols for them however a number of users have had successful results using these sample types.
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Notes
Prostaglandin E2 (PGE2) is formed in a variety of cells from PGH2, which itself is synthesized from arachidonic acid by the enzyme prostaglandin synthetase. PGE2 has been shown to have a number of biological actions, including vasodilation, both anti- and proinflammatory action, modulation of sleep/wake cycles, and facilitation of the replication of human immunodeficiency virus. It elevates cAMP levels, stimulates bone resorption, and has thermoregulatory effects. It has been shown to be a regulator of sodium excretion and renal hemodynamics.
Cross Reactivity
Compound % Cross Reactivity PGE2 100 PGE1 70 PGE3 16.3 PGF1α 1.4 PGF2α 0.7 6-keto-PGF1α 0.6 PGA2 0.1 PGB1 0.1 13,14-dihydro-15-keto-PGF2α 6,15-keto-13,14-dihydro-PGF1α Thromboxane B2 2-Arachidonoylglycerol Anandamide PGD2 Arachadonic Acid -
Platform
Microplate
Properties
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Storage instructions
Please refer to protocols. -
Components 1 x 96 tests 20X Wash Buffer Concentrate 1 x 27ml Assay Buffer 1 x 27ml Goat anti-mouse IgG Microplate (12 x 8 wells) 1 unit PGE2 Alkaline Phosphatase Conjugate 1 x 5ml Plate Sealer 1 unit pNpp Substrate 1 x 20ml Prostaglandin E2 Antibody 1 x 5ml Prostaglandin E2 Standard 1 x 500µl Stop Solution 1 x 5ml -
Research areas
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Relevance
Receptor for prostaglandin E2 (PGE2). The activity of this receptor is mediated by G(s) proteins that stimulate adenylate cyclase. The subsequent raise in intracellular cAMP is responsible for the relaxing effect of this receptor on smooth muscle. -
Cellular localization
Cell Membrane -
Alternative names
- EP2
- PG E2
- PGE 2
see all
Images
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Competitive ELISA - Prostaglandin E2 ELISA Kit (ab133021)Standard curve: mean of duplicates (+/- SD) with background reads subtracted
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Competitive ELISA - Prostaglandin E2 ELISA Kit (ab133021)Prostaglandin E2 measured in biological fluids and cell culture supernatants showing quantity (pg) per mL of tested sample. Samples were diluted 1-9 fold.
