Prostaglandin E2 ELISA Kit (High Sensitivity ) (ab133055)
Key features and details
- Sensitivity: 8.26 pg/ml
- Range: 7.8 pg/ml - 1000 pg/ml
- Sample type: Cell culture supernatant, EDTA Plasma, Plasma, Saliva, Serum, Urine
- Detection method: Colorimetric
- Assay type: Competitive
- Reacts with: Human
Overview
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Product name
Prostaglandin E2 ELISA Kit (High Sensitivity )
See all PGE2 kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Low conc 24 9.8% Medium conc 24 6.1% High Conc 24 3.1% Inter-assay Sample n Mean SD CV% Low conc 8 12.1% Medium conc 8 12.6% High conc 8 8.1% -
Sample type
Cell culture supernatant, Saliva, Urine, Serum, Plasma, EDTA Plasma -
Assay type
Competitive -
Sensitivity
8.26 pg/ml -
Range
7.8 pg/ml - 1000 pg/ml -
Recovery
Sample specific recovery Sample type Average % Range Saliva 123.3 % - % Urine 108.9 % - % Whole Blood 101.2 % - % Tissue Culture Media 104.4 % - % Male Serum 126.1 % - % Female Serum 113.7 % - % -
Assay time
3h 00m -
Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Human
Predicted to work with: a wide range of other species
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Product overview
Abcam’s Prostaglandin E2 High Sensitivity in vitro competitive ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate quantitative measurement of Prostaglandin E2 in serum, saliva, urine, tissue culture media and other biological fluids.
A goat anti-mouse IgG antibody has been precoated onto 96-well plates. Standards or test samples are added to the wells, along with an alkaline phosphatase (AP) conjugated-PGE2 antigen and a monoclonal mouse antibody specific to PGE2. After incubation the excess reagents are washed away. pNpp substrate is added and after a short incubation the enzyme reaction is stopped and the yellow color generated is read at 405 nm. The intensity of the yellow coloration is inversely proportional to the amount of PGE2 captured in the plate.
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Notes
Prostaglandin E2 (PGE2) is an extensively studied prostaglandin owing to its predominance in inflammation, cancer, atherosclerosis, autoimmune disease, and sepsis. Oxidation of arachidonic acid by prostaglandin synthases (COX-1 and COX-2) produces prostaglandin H2 (PGH2), which is further metabolized by PGE synthases into its major product, PGE2. PGE2 mediates autocrine and paracrine signaling by binding to G-protein coupled receptors (EP1, EP2, EP3, EP4) on the cell surface, functioning to modulate phospholipase C and adenylate cyclase activity. PGE2 has been of great interest as a therapeutic target, either by modulation of its synthesis by COX inhibitors (NSAIDS) or by modulation of its receptors by downregulation or binding antagonists. PGE2 production in a variety of tissues has been shown to modulate numerous physiological processes including natriuresis in the kidney, smooth muscle elasticity in the vasculature, and the inflammatory response to damaged tissues by monocytes and macrophages.
Compound Cross Reactivity PGE2 100% PGE1 70% PGE3 16.3% PGF1α 1.4% PGF2α 0.7% 6-keto-PGF1α 0.6% PGA2 0.1% PGB1 0.1% 13,14-dihydro-15-keto-PGF2α 6,15-keto-13,14-dihydro-PGF1α Thromboxane B2 2-Arachidonoylglycerol Anandamide PGD2 Arachadonic Acid -
Platform
Microplate
Properties
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Storage instructions
Please refer to protocols. -
Components 1 x 96 tests 20X Wash Buffer Concentrate 1 x 30ml Assay Buffer 1 x 30ml Goat anti-mouse IgG Microplate (12 x 8 wells) 1 unit PGE2 Alkaline Phosphatase Conjugate 1 x 6ml Plate Sealer 2 units pNpp Substrate 1 x 20ml Prostaglandin E2 HS Antibody 1 x 6ml Prostaglandin E2 Standard 1 x 0.5ml Stop Solution 1 x 5ml -
Research areas
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Relevance
Receptor for prostaglandin E2 (PGE2). The activity of this receptor is mediated by G(s) proteins that stimulate adenylate cyclase. The subsequent raise in intracellular cAMP is responsible for the relaxing effect of this receptor on smooth muscle. -
Cellular localization
Cell Membrane -
Alternative names
- EP2
- PG E2
- PGE 2
see all -
Database links
- Entrez Gene: 5732 Human
- Omim: 176804 Human
- SwissProt: P43116 Human
- Unigene: 2090 Human
Images
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ab133055 Prostaglandin E2 High Sensitivity ELISA Kit
PGE2 measured in human biological fluids in different ranges of dilutions (serum and EDTA-plasma at 1:5-1:50; saliva 1:1-1:5); data shown represents duplicates +/- SD. Breast milk, diluted 1:30, fell above the standard curve.
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ab133055 Prostaglandin E2 High Sensitivity ELISA Kit -
ab133055 Prostaglandin E2 High Sensitivity ELISA KitPGE2 measured in cell culture medium (dilutions range 1-1:5; duplicates; +/- SD).
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Typical Standard CurveRepresentative Standard Curve using ab133055.
