Anti-GPCR RDC1/CXCR-7 antibody (ab38089)
Key features and details
- Rabbit polyclonal to GPCR RDC1/CXCR-7
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-GPCR RDC1/CXCR-7 antibody
See all GPCR RDC1/CXCR-7 primary antibodies -
Description
Rabbit polyclonal to GPCR RDC1/CXCR-7 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB Human
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Immunogen
Synthetic peptide corresponding to Human GPCR RDC1/CXCR-7 aa 1-100 conjugated to keyhole limpet haemocyanin.
(Peptide available asab38088)
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration
Concentration information loading...Purity
Immunogen affinity purifiedClonality
PolyclonalIsotype
IgGResearch areas
Associated products
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Compatible Secondaries
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Immunizing Peptide (Blocking)
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab38089 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
GuaranteedTested applications are guaranteed to work and covered by our Abpromise guarantee.
PredictedPredicted to work for this combination of applications and species but not guaranteed.
IncompatibleDoes not work for this combination of applications and species.
Application Species WB HumanAll applications MouseApplication Abreviews Notes WB (2) 1/250. Detects a band of approximately 52 kDa (predicted molecular weight: 42 kDa).Can be blocked with Human GPCR RDC1/CXCR-7 peptide (ab38088).GPCR RCD1 is glycosylated so it is not unexpected that it runs higher than predicted (based on primary structure) in SDS PAGE.
Notes WB
1/250. Detects a band of approximately 52 kDa (predicted molecular weight: 42 kDa).Can be blocked with Human GPCR RDC1/CXCR-7 peptide (ab38088).GPCR RCD1 is glycosylated so it is not unexpected that it runs higher than predicted (based on primary structure) in SDS PAGE.
Target
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Function
Receptor for CXCL12/SDF1. Acts as coreceptor with CXCR4 for a restricted number of HIV isolates. -
Tissue specificity
Expressed in monocytes, basophils, and B-cells. Lower expression in CD4+ T-lymphocytes and natural killer cells. -
Sequence similarities
Belongs to the G-protein coupled receptor 1 family. -
Cellular localization
Cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 57007 Human
- Entrez Gene: 12778 Mouse
- Omim: 610376 Human
- SwissProt: P25106 Human
- SwissProt: P56485 Mouse
- Unigene: 471751 Human
- Unigene: 6522 Mouse
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Alternative names
- ACKR3 antibody
- atypical chemokine receptor 3 antibody
- C X C chemokine receptor type 7 antibody
see all
Images
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Western blot - Anti-GPCR RDC1/CXCR-7 antibody (ab38089)All lanes : Anti-GPCR RDC1/CXCR-7 antibody (ab38089) at 1/250 dilution
Lane 1 : Human Brain Tissue Lysate
Lane 2 : Human Testis Tissue Lysate
Lane 3 : Human Liver Tissue Lysate
Lane 4 : Human Heart Tissue Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution
Performed under reducing conditions.
Predicted band size: 42 kDa
Observed band size: 52 kDa why is the actual band size different from the predicted?
GPCR RCD1 is glycosylated so it is not unexpected that it runs higher than predicted (based on primary structure) in SDS PAGE.
Datasheets and documents
References (22)
ab38089 has been referenced in 22 publications.
- Zhang L et al. Cytokine augments the sorafenib-induced apoptosis in Huh7 liver cancer cell by inducing mitochondrial fragmentation and activating MAPK-JNK signalling pathway. Biomed Pharmacother 110:213-223 (2019). PubMed: 30476722
- Küçükköse C & Yalçin Özuysal Ö Effects of Notch signalling on the expression of SEMA3C, HMGA2, CXCL14, CXCR7, and CCL20 in breast cancer. Turk J Biol 43:70-76 (2019). PubMed: 30930637
- Zhang J et al. Overexpression of macrophage stimulating 1 enhances the anti-tumor effects of IL-24 in esophageal cancer via inhibiting ERK-Mfn2 signaling-dependent mitophagy. Biomed Pharmacother 114:108844 (2019). PubMed: 30981108
- Bai Y et al. RUNX2 overexpression and PTEN haploinsufficiency cooperate to promote CXCR7 expression and cellular trafficking, AKT hyperactivation and prostate tumorigenesis. Theranostics 9:3459-3475 (2019). PubMed: 31281490
- Kim N et al. CXCR7 promotes migration and invasion in head and neck squamous cell carcinoma by upregulating TGF-ß1/Smad2/3 signaling. Sci Rep 9:18100 (2019). PubMed: 31792315
Images
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Western blot - Anti-GPCR RDC1/CXCR-7 antibody (ab38089)All lanes : Anti-GPCR RDC1/CXCR-7 antibody (ab38089) at 1/250 dilution
Lane 1 : Human Brain Tissue Lysate
Lane 2 : Human Testis Tissue Lysate
Lane 3 : Human Liver Tissue Lysate
Lane 4 : Human Heart Tissue Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution
Performed under reducing conditions.
Predicted band size: 42 kDa
Observed band size: 52 kDa why is the actual band size different from the predicted?
GPCR RCD1 is glycosylated so it is not unexpected that it runs higher than predicted (based on primary structure) in SDS PAGE.
