Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-P2Y12 antibody [EPR23511-72] - BSA and Azide free (ab274386)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR23511-72] to P2Y12 - BSA and Azide free
  • Suitable for: IHC-P, Dot blot
  • Reacts with: Human

Overview

  • Product name

    Anti-P2Y12 antibody [EPR23511-72] - BSA and Azide free
    See all P2Y12 primary antibodies

  • Description

    Rabbit monoclonal [EPR23511-72] to P2Y12 - BSA and Azide free

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Dot
    Human
    IHC-P
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • IHC-P: Human cerebrum and glioma tissue.

  • General notes

    ab274386 is the carrier-free version of ab254347. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-P2Y12 antibody [EPR23511-72] - BSA and Azide free (ab274386)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-P2Y12 antibody [EPR23511-72] - BSA and Azide free (ab274386)

    This data was developed using ab254347, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded Human glioma tissue labeling P2Y12 with ab254347 at 1/1000 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining in human glioma (PMID: 30832693). The section was incubated with ab254347 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

  • Dot Blot - Anti-P2Y12 antibody [EPR23511-72] - BSA and Azide free (ab274386)
    Dot Blot - Anti-P2Y12 antibody [EPR23511-72] - BSA and Azide free (ab274386)

    This data was developed using ab254347, the same antibody clone in a different buffer formulation.

    Dot blot analysis of P2Y12 using ab254347 at 1/1000 dilution (0.569 ug/ml) followed by a Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/100,000 dilution.

    Lane 1: Human P2Y12 immunogen peptide (aa 162-181)

    Lane 2: Human P2Y12 non-immunogen peptide (aa 323-340)

    Lane 3: Human P2Y12 non-immunogen peptide (aa 308-328)

    Lane 4: BSA

    Exposure time: 3 minutes

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-P2Y12 antibody [EPR23511-72] - BSA and Azide free (ab274386)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-P2Y12 antibody [EPR23511-72] - BSA and Azide free (ab274386)

    This data was developed using ab254347, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded Human cerebrum tissue labeling P2Y12 with ab254347 at 1/1000 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on microglia (PMID: 30832693). The section was incubated with ab254347 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

  • Anti-P2Y12 antibody [EPR23511-72] - BSA and Azide free (ab274386)
    Anti-P2Y12 antibody [EPR23511-72] - BSA and Azide free (ab274386)

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