Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-LGR5 antibody [EPR3065Y] - BSA and Azide free (ab199335)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR3065Y] to LGR5 - BSA and Azide free
  • Suitable for: WB
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-LGR5 antibody [EPR3065Y] - BSA and Azide free
    See all LGR5 primary antibodies

  • Description

    Rabbit monoclonal [EPR3065Y] to LGR5 - BSA and Azide free

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    WB
    Mouse
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Fetal skeletal, human and mouse skeletal, mouse and rat brain lysates.

  • General notes

    Ab199335 is the carrier-free version of ab75850. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab199335 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

Images

  • Western blot - Anti-LGR5 antibody [EPR3065Y] - BSA and Azide free (ab199335)
    Western blot - Anti-LGR5 antibody [EPR3065Y] - BSA and Azide free (ab199335)
    Anti-LGR5 antibody [EPR3065Y] (ab75850) at 1/1000 dilution (Purified) + Human skeletal muscle lysate at 20 µg

    Secondary
    Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

    Predicted band size: 100 kDa
    Observed band size: 100 kDa



    This data was developed using ab75850, the same antibody clone in a different buffer formulation. 

    Blocking Buffer and concentration: 5% NFDM/TBST

  • Western blot - Anti-LGR5 antibody [EPR3065Y] - BSA and Azide free (ab199335)
    Western blot - Anti-LGR5 antibody [EPR3065Y] - BSA and Azide free (ab199335)
    All lanes : Anti-LGR5 antibody [EPR3065Y] (ab75850) at 1/1000 dilution (Purified)

    Lane 1 : Mouse skeletal muscle lysate
    Lane 2 : Rat skeletal muscle lysate
    Lane 3 : Mouse brain lysate
    Lane 4 : Rat brain lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

    Predicted band size: 100 kDa
    Observed band size: 100 kDa
    Additional bands at: 38 kDa. We are unsure as to the identity of these extra bands.



    This data was developed using ab75850, the same antibody clone in a different buffer formulation.

    We are unsure about the identity of the additional band.

    Blocking Buffer and concentration: 5% NFDM/TBST

  • Western blot - Anti-LGR5 antibody [EPR3065Y] - BSA and Azide free (ab199335)
    Western blot - Anti-LGR5 antibody [EPR3065Y] - BSA and Azide free (ab199335)
    Anti-LGR5 antibody [EPR3065Y] (ab75850) at 1/1000 dilution (unpurified) + Fetal skeletal muscle tissue lysate at 10 µg

    Secondary
    HRP-labeled goat anti-rabbit at 1/2000 dilution

    Predicted band size: 100 kDa
    Observed band size: 100 kDa



    This data was developed using ab75850, the same antibody clone in a different buffer formulation.

  • Anti-LGR5 antibody [EPR3065Y] - BSA and Azide free (ab199335)
    Anti-LGR5 antibody [EPR3065Y] - BSA and Azide free (ab199335)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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