Anti-Japanese encephalitis virus NS1 glycoprotein antibody [JN1] (ab41651)
![Anti-Japanese encephalitis virus NS1 glycoprotein antibody [JN1] (ab41651)](https://www.abcam.com/ps/products/41/ab41651/Images/ab41651-55009-anti-japanese-encephalitis-virus-ns1-glycoprotein-antibody-jn1-immunofluorescence.jpg "Anti-Japanese encephalitis virus NS1 glycoprotein antibody [JN1] (ab41651)")
Key features and details
- Mouse monoclonal [JN1] to Japanese encephalitis virus NS1 glycoprotein
- Suitable for: WB, ICC/IF, ELISA
- Reacts with: Japanese encephalitis virus
- Isotype: IgG3
Overview
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Product name
Anti-Japanese encephalitis virus NS1 glycoprotein antibody [JN1]
See all Japanese encephalitis virus NS1 glycoprotein primary antibodies -
Description
Mouse monoclonal [JN1] to Japanese encephalitis virus NS1 glycoprotein -
Host species
Mouse -
Tested applications
Suitable for: WB, ICC/IF, ELISAmore details -
Species reactivity
Reacts with: Japanese encephalitis virus -
Immunogen
Full length native protein purified from Japanese encephalitis virus (Nakayama) supernatant
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.065% Sodium azide -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
JN1 -
Isotype
IgG3 -
Light chain type
kappa -
Research areas
Images
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Immunocytochemistry/ Immunofluorescence - Anti-Japanese encephalitis virus NS1 glycoprotein antibody [JN1] (ab41651)Japanese encephalitis virus (Nakayama) infected PS clone D cells stained with ab41651 (green).
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![Western blot - Anti-Japanese encephalitis virus NS1 glycoprotein antibody [JN1] (ab41651)](https://www.abcam.com/ps/products/41/ab41651/Images/ab41651-55008-anti-japanese-encephalitis-virus-ns1-glycoprotein-antibody-jn1-western-blot.jpg)
Western blot - Anti-Japanese encephalitis virus NS1 glycoprotein antibody [JN1] (ab41651)All lanes : Anti-Japanese encephalitis virus NS1 glycoprotein antibody [JN1] (ab41651)
Lane 1 : Japanese encephalitis virus infected C6/36 cell lysate (unheated)
Lane 2 : Japanese encephalitis virus infected C6/36 cell lysate (boiled)
Predicted band size: 46 kDa
Observed band size: 46,92 kDa why is the actual band size different from the predicted?
Additional bands at: 100 kDa (possible cleavage fragment), 50 kDa (possible cleavage fragment)
This antibody recognsies 2 forms of NS1 - NS1 and NS1' (46 and 53 kDa respectively). NS1' is thought to be formed when NS1 is cleaved from NS2A at an alternative site. Both NS1 and NS1' exist as dimers in untreated samples but are dissociated into monomers when samples are boiled.