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Signal Transduction Signaling Pathway G Protein Signaling GPCR

Anti-LGR5 antibody [EPR3065Y] (ab75850)

Price and availability

355 142 ₸

Availability

Order now and get it on Tuesday March 02, 2021

Anti-LGR5 antibody [EPR3065Y] (ab75850)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR3065Y] to LGR5
  • Suitable for: WB
  • Reacts with: Mouse, Rat, Human

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Overview

  • Product name

    Anti-LGR5 antibody [EPR3065Y]
    See all LGR5 primary antibodies
  • Description

    Rabbit monoclonal [EPR3065Y] to LGR5
  • Host species

    Rabbit
  • Tested Applications & Species

    Application Species
    WB
    Mouse
    Rat
    Human
    See all applications and species data
  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Fetal skeletal, human and mouse skeletal, mouse and rat brain lysates.
  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 0.05% BSA, 40% Glycerol (glycerin, glycerine), 59% PBS
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR3065Y
  • Isotype

    IgG
  • Research areas

    • Signal Transduction
    • Signaling Pathway
    • G Protein Signaling
    • GPCR
    • Stem Cells
    • Signaling Pathways
    • Wnt
    • Surface Molecules
    • Cancer
    • Signal transduction
    • G protein signaling
    • GPCR

Images

  • Western blot - Anti-LGR5 antibody [EPR3065Y] (ab75850)
    Western blot - Anti-LGR5 antibody [EPR3065Y] (ab75850)
    Anti-LGR5 antibody [EPR3065Y] (ab75850) at 1/1000 dilution (Purified) + Human skeletal muscle lysate at 20 µg

    Secondary
    Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

    Predicted band size: 100 kDa
    Observed band size: 100 kDa



    Blocking Buffer and concentration: 5% NFDM/TBST

  • Western blot - Anti-LGR5 antibody [EPR3065Y] (ab75850)
    Western blot - Anti-LGR5 antibody [EPR3065Y] (ab75850)
    All lanes : Anti-LGR5 antibody [EPR3065Y] (ab75850) at 1/1000 dilution (Purified)

    Lane 1 : Mouse skeletal muscle lysate
    Lane 2 : Rat skeletal muscle lysate
    Lane 3 : Mouse brain lysate
    Lane 4 : Rat brain lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

    Predicted band size: 100 kDa
    Observed band size: 100 kDa
    Additional bands at: 38 kDa. We are unsure as to the identity of these extra bands.



    We are unsure about the identity of the additional band.

    Blocking Buffer and concentration: 5% NFDM/TBST

  • Western blot - Anti-LGR5 antibody [EPR3065Y] (ab75850)
    Western blot - Anti-LGR5 antibody [EPR3065Y] (ab75850)
    Anti-LGR5 antibody [EPR3065Y] (ab75850) at 1/1000 dilution (unpurified) + Fetal
    skeletal muscle lysate at 10 µg

    Secondary
    HRP-labeled goat anti-rabbit at 1/2000 dilution

    Predicted band size: 100 kDa
    Observed band size: 100 kDa

  • Anti-LGR5 antibody [EPR3065Y] (ab75850)
    Anti-LGR5 antibody [EPR3065Y] (ab75850)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Alternative products to Anti-LGR5 antibody [EPR3065Y] (ab75850)

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    Anti-LGR5 antibody (ab261734)

    Applications: IHC-P

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    Anti-LGR5 antibody [1A2G2] (ab238518)

    Applications: WB

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    Anti-LGR5 antibody (ab237598)

    Applications: WB

  •  
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    Anti-LGR5 antibody (ab219107)

    Applications: IHC-P

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    Anti-LGR5 antibody [EPR3065Y] - BSA and Azide free (ab199335)

    Applications: WB

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