Human GASP-1 ELISA Kit (ab267602)
Key features and details
- Sensitivity: 20 pg/ml
- Range: 20.48 pg/ml - 5000 pg/ml
- Sample type: Cell culture media, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Overview
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Product name
Human GASP-1 ELISA Kit -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Overall Inter-assay Sample n Mean SD CV% Overall -
Sample type
Serum, Plasma, Cell culture media -
Assay type
Sandwich (quantitative) -
Sensitivity
20 pg/ml -
Range
20.48 pg/ml - 5000 pg/ml -
Recovery
Sample specific recovery Sample type Average % Range Serum 108 92% - 127% Plasma 101 89% - 123% Cell culture media 94 80% - 108% -
Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Human -
Product overview
Human GASP-1 ELISA Kit is designed for the quantitative determination of GASP-1 in cell culture supernatants, plasma and serum samples.
This assay employs an antibody specific for human GASP-1 coated on a 96-well plate. Standards and samples are pipetted into the wells and GASP-1 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-human GASP-1 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of GASP-1 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
Cross Reactivity: This ELISA kit shows no cross-reactivity with the following cytokines tested: human ACE-2, aFGF, Albumin, AMICA, Angiopoietin-4, BAFF, CA19-9, CD163, Clusterin, CRTAM, CXCL14, Cystatin C, Decorin, Dkk-3, DLL1, Fetuin A, FOLR1, Furin, GASP-2, GCSF R, HAI-2, IL-17 RB, IL-27, LAG-3, LDL R, Pepsinogen 1, RANK, RBP4, SOST, Syndecan-1, TACI, TFPI, Thrombospondin 1, TRAIL R2, TRANCE, Troponin I, uPA, VECadherin, WISP-1.
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Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 1 x 96 tests 200X HRP-Streptavidin Concentrate 1 x 200µl 20X Wash Buffer 1 x 25ml 5X Assay Diluent 1 x 15ml Anti-Human GASP-1 coated Microplate (12 x 8 wells) 1 unit Biotinylated Anti-Human GASP-1 Detection Antibody 2 vials Human GASP-1 Standard (Lyophilized) 2 vials Stop Solution 1 x 8ml TMB Substrate Solution 1 x 12ml -
Research areas
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Function
May modulate lysosomal sorting and functional down-regulation of a variety of G-protein coupled receptors. Targets receptors for degradation in lysosomes. -
Tissue specificity
Expressed in the brain, with lower expression in medulla, spinal cord and substantia nigra. -
Sequence similarities
Belongs to the GPRASP family. -
Cellular localization
Cytoplasm. - Information by UniProt
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Alternative names
- G-protein coupled receptor-associated sorting protein 1
- GASP
- GASP-1
see all -
Database links
- Entrez Gene: 9737 Human
- Omim: 300417 Human
- SwissProt: Q5JY77 Human
- Unigene: 522729 Human
Images
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Example standard curve
Human GASP-1 Standard curve.
These standard curves are for demonstration only. A standard curve must be run with each assay