Anti-BOP1 antibody (ab86652)
Key features and details
- Rabbit polyclonal to BOP1
- Suitable for: IP, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-BOP1 antibody
See all BOP1 primary antibodies -
Description
Rabbit polyclonal to BOP1 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IHC-P MouseHumanIP Human
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Immunogen
Synthetic peptide corresponding to a region between residue 350 and 400 of Human BOP1 (NP_056016.1).
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BOP1 antibody (ab86652)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human colon carcinoma (left) and mouse teratoma tissues labelling BOP1 with ab86652 at 1/200 (1µg/ml). Detection: DAB.
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Immunoprecipitation - Anti-BOP1 antibody (ab86652)Western Blot detection of BOP1 in Immunoprecipitates of HeLa whole cell lysate (1 mg for IP, 20% of IP loaded) using ab86652 at 3 µg/mg lysate; Lane 2 represents control IgG IP. For the WB, ab86653 was used at 0.4 µg/ml.
Detection: Chemiluminescence with exposure time of 90 seconds.
All lanes : Anti-BOP1 antibody (ab86652) at 0.4 µg/ml
Lane 1 : immunoprecipitated BOP1
Lane 2 : control IgG
Observed band size: 101 kDa why is the actual band size different from the predicted?
Additional bands at: 200 kDa, 55 kDa, 60-71 kDa. We are unsure as to the identity of these extra bands.
