Anti-EIF3H antibody (ab251743)
Key features and details
- Rabbit polyclonal to EIF3H
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-EIF3H antibody
See all EIF3H primary antibodies -
Description
Rabbit polyclonal to EIF3H -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Rat, Human
Predicted to work with: Cow
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Immunogen
Recombinant fragment corresponding to Human EIF3H aa 250 to the C-terminus.
Database link: O15372 -
Positive control
- IHC-P: Human duodenum, cervix, testis and cerebellum tissue. ICC/IF: U-2 OS cells. WB: NIH/3T3, NBT-II, RT$ and U-251 MG whole cell lysates; human liver and tonsil lysates.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Immunocytochemistry/ Immunofluorescence - Anti-EIF3H antibody (ab251743)PFA-fixed, Triton X-100 permeabilized U-2 OS (human bone osteosarcoma epithelial cell line) cells stained for EIF3H (green) using ab251743 at 4 µg/ml in ICC/IF.
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Western blot - Anti-EIF3H antibody (ab251743)All lanes : Anti-EIF3H antibody (ab251743) at 0.4 µg/ml
Lane 1 : NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate
Lane 2 : NBT-II (rat Wistar bladder tumor cell line) whole cell lysate
Predicted band size: 40 kDa
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EIF3H antibody (ab251743)Paraffin-embedded human pancreas tissue stained for EIF3H using ab251743 at 1/1000 dilution in immunohistochemical analysis.
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Western blot - Anti-EIF3H antibody (ab251743)All lanes : Anti-EIF3H antibody (ab251743) at 0.4 µg/ml
Lane 1 : RT4 (human urinary bladder cancer cell line) whole cell lysate
Lane 2 : U-251 MG (human brain glioma cell line) whole cell lysate
Lane 3 : Human plasma (IgG/HSA depleted)
Lane 4 : Human liver lysate
Lane 5 : Human tonsil lysate
Predicted band size: 40 kDa