Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Endothelin A Receptor/ET-A antibody [UMB-8-37-1] - BSA and Azide free (ab242440)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [UMB-8-37-1] to Endothelin A Receptor/ET-A - BSA and Azide free
  • Suitable for: IHC-P, WB
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-Endothelin A Receptor/ET-A antibody [UMB-8-37-1] - BSA and Azide free
    See all Endothelin A Receptor/ET-A primary antibodies

  • Description

    Rabbit monoclonal [UMB-8-37-1] to Endothelin A Receptor/ET-A - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: IHC-P, WBmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Mouse heart and lung tissue lysates; IHC-P: Mouse and rat cardiac muscle and human placenta tissue.

  • General notes

    ab242440 is a PBS-only buffer format of ab178454. Please refer to ab178454 for recommended dilutions, protocols, and image data.

    This product has switched from a hybridoma to recombinant production method on 9th September 2020.

    The formulation and the concentration of this product is compatible for metal-conjugation for mass cytometry (CyTOF®).

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-Endothelin A Receptor/ET-A antibody [UMB-8-37-1] - BSA and Azide free (ab242440)
    Western blot - Anti-Endothelin A Receptor/ET-A antibody [UMB-8-37-1] - BSA and Azide free (ab242440)
    All lanes : Anti-Endothelin A Receptor/ET-A antibody [UMB-8-37-1] (ab178454) at 1/1000 dilution

    Lane 1 : Mouse heart lysate
    Lane 2 : Mouse lung lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 49 kDa
    Observed band size: 40-70 kDa
    why is the actual band size different from the predicted?


    Exposure time: 26 seconds


    This data was developed using the same antibody clone in a different buffer formulation (ab178454).

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Endothelin A Receptor/ET-A antibody [UMB-8-37-1] - BSA and Azide free (ab242440)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Endothelin A Receptor/ET-A antibody [UMB-8-37-1] - BSA and Azide free (ab242440)

    This data was developed using the same antibody clone in a different buffer formulation (ab178454).

    Immunohistochemical analysis of paraffin-embedded human placenta tissue labeling Endothelin A Receptor/ET-A with ab178454 at 1/100 dilution, followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Cytoplasmic staining on stroma cells of human ovarian carcinoma is observed. Counterstained with hematoxylin. Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Heat mediated antigen retrieval using ab208572 (Universal HIER antigen retrieval reagent).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Endothelin A Receptor/ET-A antibody [UMB-8-37-1] - BSA and Azide free (ab242440)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Endothelin A Receptor/ET-A antibody [UMB-8-37-1] - BSA and Azide free (ab242440)

    This data was developed using the same antibody clone in a different buffer formulation (ab178454).

    Immunohistochemical analysis of paraffin-embedded rat cardiac muscle tissue labeling Endothelin A Receptor/ET-A with ab178454 at 1/100 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Positive staining on rat cardiac muscle is observed. Counterstained with hematoxylin. Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP). Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Endothelin A Receptor/ET-A antibody [UMB-8-37-1] - BSA and Azide free (ab242440)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Endothelin A Receptor/ET-A antibody [UMB-8-37-1] - BSA and Azide free (ab242440)

    This data was developed using the same antibody clone in a different buffer formulation (ab178454).

    Immunohistochemical analysis of paraffin-embedded mouse cardiac muscle tissue labeling Endothelin A Receptor/ET-A with ab178454 at 1/100 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Positive staining on mouse cardiac muscle is observed. Counterstained with hematoxylin. Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP). Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

  • Western blot - Anti-Endothelin A Receptor/ET-A antibody [UMB-8-37-1] - BSA and Azide free (ab242440)
    Western blot - Anti-Endothelin A Receptor/ET-A antibody [UMB-8-37-1] - BSA and Azide free (ab242440)
    All lanes : Anti-Endothelin A Receptor/ET-A antibody [UMB-8-37-1] (ab178454) at 1/1000 dilution

    Lane 1 : HEK-293 (human embryonic kidney) transfected with an empty vector (vector control), containing a myc-His-tag®, whole cell lysate
    Lane 2 : HEK-293 transfected with Endothelin A Receptor/ET-A (WT) expression vector containing a myc-His-tag® , whole cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 49 kDa
    Observed band size: 40-80 kDa why is the actual band size different from the predicted?


    Exposure time: 3 minutes


    This data was developed using the same antibody clone in a different buffer formulation (ab178454).

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

  • Anti-Endothelin A Receptor/ET-A antibody [UMB-8-37-1] - BSA and Azide free (ab242440)
    Anti-Endothelin A Receptor/ET-A antibody [UMB-8-37-1] - BSA and Azide free (ab242440)

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