TRAP-6, PAR1 agonist (ab120801)
Key features and details
- PAR1 agonist
- CAS Number: 141136-83-6
- Purity: > 95%
Soluble in water to 1 mg/ml
- Form / State: Solid
- Source: Synthetic
Overview
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Product name
TRAP-6, PAR1 agonist -
Description
PAR1 agonist -
Alternative names
- Thrombin Receptor Activator Peptide 6
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Biological description
Peptide fragment (residues 42 - 47) of protease-activated receptor 1. PAR1 hexapeptide agonist. Stimulates transient mobilization of free intracellular calcium, platelet aggregation (EC50 = 0.8μM) and rapidly phosphorylates PDE3A. -
Purity
> 95% -
CAS Number
141136-83-6 -
Chemical structure
Properties
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Molecular weight
748.88 -
Molecular formula
C34H56N10O9 -
Sequence
SFLLRN -
Storage instructions
Store at -20°C. Store under desiccating conditions. The product can be stored for up to 12 months. -
Solubility overview
Soluble in water to 1 mg/ml
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Handling
Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20°C. Generally, these will be useable for up to one week. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.
Need more advice on solubility, usage and handling? Please visit our frequently asked questions (FAQ) page for more details.
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Source
Synthetic
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Research areas
Images
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Functional Studies - TRAP-6, PAR1 agonist (ab120801)ab6672 staining IL6 in serum starved PC3 cells treated with TRAP-6 (ab120801), by ICC/IF. Increase in expression of IL6 correlates with increased concentration of TRAP-6, as described in literature.
The cells were incubated at 37°C for 6h in media containing different concentrations of ab120801 (TRAP-6) in DMSO, fixed with 4% formaldehyde for 10 minutes at room temperature and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab6672 (1/500 dilution) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 goat anti-rabbit polyclonal antibody (ab96899) at 1/250 dilution was used as the secondary antibody. Nuclei were counterstained with DAPI and are shown in blue.
