Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-MRO antibody [EPR12158] - BSA and Azide free (ab250308)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR12158] to MRO - BSA and Azide free
  • Suitable for: IP, WB
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-MRO antibody [EPR12158] - BSA and Azide free
    See all MRO primary antibodies

  • Description

    Rabbit monoclonal [EPR12158] to MRO - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: IP, WBmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    Ab250308 is the carrier-free version of ab181048. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab250308 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C. Do Not Freeze.

  • Storage buffer

    pH: 7.2
    Constituent: PBS

  • Carrier free

    Yes
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR12158

  • Isotype

    IgG

Images

  • Western blot - Anti-MRO antibody [EPR12158] - BSA and Azide free (ab250308)
    Western blot - Anti-MRO antibody [EPR12158] - BSA and Azide free (ab250308)
    All lanes : Anti-MRO antibody [EPR12158] (ab181048) at 1/5000 dilution (purified)

    Lane 1 : mouse spleen lysate
    Lane 2 : rat spleen lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : HRP goat anti-rabbit IgG (H+L) at 1/1000 dilution

    Predicted band size: 29 kDa
    Observed band size: 29 kDa

  • Western blot - Anti-MRO antibody [EPR12158] - BSA and Azide free (ab250308)
    Western blot - Anti-MRO antibody [EPR12158] - BSA and Azide free (ab250308)
    Anti-MRO antibody [EPR12158] (ab181048) at 1/5000 dilution (purified) + human fetal heart tissue lysate at 10 µg

    Secondary
    HRP goat anti-rabbit IgG (H+L) at 1/1000 dilution

    Predicted band size: 29 kDa
    Observed band size: 29 kDa



    This data was developed using ab181048, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

  • Western blot - Anti-MRO antibody [EPR12158] - BSA and Azide free (ab250308)
    Western blot - Anti-MRO antibody [EPR12158] - BSA and Azide free (ab250308)
    Anti-MRO antibody [EPR12158] (ab181048) at 1/5000 dilution (purified) + Human fetal brain tissue lysate at 10 µg

    Secondary
    HRP goat anti-rabbit IgG (H+L) at 1/1000 dilution

    Predicted band size: 29 kDa
    Observed band size: 29 kDa



    This data was developed using ab181048, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

  • Immunoprecipitation - Anti-MRO antibody [EPR12158] - BSA and Azide free (ab250308)
    Immunoprecipitation - Anti-MRO antibody [EPR12158] - BSA and Azide free (ab250308)
    This data was developed using ab181048, the same antibody clone in a different buffer formulation.ab181048 (purified) at 1/20 immunoprecipitating MRO in 10 μg human fetal heart whole cell lysate (Lanes 1 and 2, observed at 29 kDa). Lane 3 - PBS. For western blotting, VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10,000 dilution. Blocking buffer and concentration: 5% NFDM/TBST Dilution buffer and concentration: 5% NFDM/TBST
  • Western blot - Anti-MRO antibody [EPR12158] - BSA and Azide free (ab250308)
    Western blot - Anti-MRO antibody [EPR12158] - BSA and Azide free (ab250308)
    All lanes : Anti-MRO antibody [EPR12158] (ab181048) at 1/1000 dilution (unpurified)

    Lane 1 : Human ovary cancer lysate
    Lane 2 : Human fetal brain lysate
    Lane 3 : Human fetal heart lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab136636) at 1/500 dilution

    Predicted band size: 29 kDa



    This data was developed using ab181048, the same antibody clone in a different buffer formulation.

  • Immunoprecipitation - Anti-MRO antibody [EPR12158] - BSA and Azide free (ab250308)
    Immunoprecipitation - Anti-MRO antibody [EPR12158] - BSA and Azide free (ab250308)
    This data was developed using ab181048, the same antibody clone in a different buffer formulation.Western blot analysis of immunoprecipitation pellet from Human fetal heart lysate (lane 1) or a negative control (lane 2) immunoprecipitated using unpurified ab181048 at 1/80 dilution.
    Secondary: Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1500 dilution.
  • Anti-MRO antibody [EPR12158] - BSA and Azide free (ab250308)
    Anti-MRO antibody [EPR12158] - BSA and Azide free (ab250308)

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