Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-BRCC36 antibody [EPR4366] (ab108411)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR4366] to BRCC36
  • Suitable for: WB, Flow Cyt
  • Knockout validated
  • Reacts with: Mouse, Human

Overview

  • Product name

    Anti-BRCC36 antibody [EPR4366]
    See all BRCC36 primary antibodies

  • Description

    Rabbit monoclonal [EPR4366] to BRCC36

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: HeLa, MCF7, SKBR-3, HAP1 and 293T cell lysates Flow Cyt: permeabilized HeLa cells

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-BRCC36 antibody [EPR4366] (ab108411)
    Western blot - Anti-BRCC36 antibody [EPR4366] (ab108411)

    Lane 1: Wild type HAP1 whole cell lysate (40 µg)
    Lane 2: Empty Lane
    Lane 3: BRCC3 knockout HAP1 whole cell lysate (40 µg)
    Lane 4: MCF7 whole cell lysate (40 µg)

    Lanes 1 - 4: Merged signal (red and green). Green - ab108411 observed at 36 kDa. Red - loading control, ab18058, observed at 130 kDa.

    ab108411 was shown to recognize empty when empty knockout samples were used, along with additional cross-reactive bands. Wild-type and empty knockout samples were subjected to SDS-PAGE.  Ab108411 and ab18058 (Mouse anti Vinculin loading control) were incubated overnight at 4°C at 10000 dilution and 1/10000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.

  • Western blot - Anti-BRCC36 antibody [EPR4366] (ab108411)
    Western blot - Anti-BRCC36 antibody [EPR4366] (ab108411)
    All lanes : Anti-BRCC36 antibody [EPR4366] (ab108411) at 1/10000 dilution

    Lane 1 : HeLa cell lysate
    Lane 2 : MCF7 cell lysate
    Lane 3 : SKBR-3 cell lysate
    Lane 4 : 293T cell lysate

    Lysates/proteins at 10 µg per lane.

    Predicted band size: 36 kDa

  • Flow Cytometry - Anti-BRCC36 antibody [EPR4366] (ab108411)
    Flow Cytometry - Anti-BRCC36 antibody [EPR4366] (ab108411)
    Flow cytometric analysis of BRCC36 in permeabilized HeLa cells, using ab108411 at a 1/100 dilution (red) or a Rabbit IgG (negative) (green).
  • Anti-BRCC36 antibody [EPR4366] (ab108411)
    Anti-BRCC36 antibody [EPR4366] (ab108411)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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