Bile Acid Assay Kit (Colorimetric) (ab239702)
Key features and details
- Detection method: Colorimetric
- Platform: Microplate reader
- Assay time: 1 hr 20 min
- Sample type: Plasma, Saliva, Serum, Urine
Overview
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Product name
Bile Acid Assay Kit (Colorimetric) -
Detection method
Colorimetric -
Sample type
Saliva, Urine, Serum, Plasma -
Assay time
1h 20m -
Product overview
Bile Acid Assay Kit (Colorimetric) ab239702 provides a simple, sensitive, and high-throughput suitable approach to quantify the concentration of total bile acid in biological fluids.
The bile acid assay is based on an enzymatic cycling method in the presence of NADH and a chromophore. The reduction of the chromophore produces a stable colorimetric product the absorbance of which can be followed kinetically at 405 nm on a multi-well spectrophotometer. This absorbance is directly proportional to the amount of total bile acids in the sample.
Other metabolites found in biofluids do not interfere with the assay.
The assay can detect as little as 1 µM of Bile Acids in a variety of samples.
Bile acid assay protocol summary:
- add samples and standards to wells
- add probe mix and incubate at 37°C for 10 min
- add reaction mix
- measure absorbance at 405 nm for 60 min at 37°C in a kinetic mode -
Platform
Microplate reader
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 100 tests NADH 1 vial TBA Cycling Assay Buffer 1 x 7ml TBA Cycling Enzyme Mix 1 vial TBA Probe 1 vial TBA Probe Buffer 1 x 14ml TBA Standard (100 mM) 1 vial -
Relevance
Bile Acids (BA) make 67% of the total composition of Bile. They are 24-carbon steroids generated during cholesterol metabolism. They form conjugates with either glycine or taurine to form bile salts. Five of the BAs account for more than 99% of the total population found in biofluids. The average composition in healthy individuals includes conjugates of cholic, chenodeoxycholic, deoxycholic and lithocholic acids. Bile acids are critical due to their ability to solubilize lipids by forming micelles with cholesterol, and fatty acids. Their synthesis is not only critical for the removal of cholesterol from the body abut they are also needed for proper uptake of dietary lipids into the small intestine.
Images
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Bile Acid Assay Standard Curve.
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Estimation of Bile Acid concentration in human serum and urine.30 µL of each undiluted sample was assayed following the kit protocol. Bile Acids concentrations are: Serum (in µM): A: 3.56 ± 0.41, B: 2.41 ± 0.27, C: 1.63 ± 0.17, D: 1.34 ± 0.25, Urine: 0.16 ± 0.02 µM/mM Creatinine.