Phosphoenolpyruvic acid (PEP) Assay Kit (ab204713)
Key features and details
- Assay type: Enzyme activity
- Detection method: Colorimetric/Fluorometric
- Platform: Microplate reader
- Sample type: Cell Lysate, Other biological fluids, Plasma, Serum, Tissue Lysate, Urine
- Sensitivity: 1 µM
Overview
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Product name
Phosphoenolpyruvic acid (PEP) Assay Kit -
Detection method
Colorimetric/Fluorometric -
Sample type
Urine, Serum, Plasma, Other biological fluids, Cell Lysate, Tissue Lysate -
Assay type
Enzyme activity -
Sensitivity
1 µM -
Product overview
Phosphoenolpyruvic acid (PEP) Assay Kit (ab204713) provides a convenient colorimetric and fluorometric means to measure PEP levels in various samples. In the assay, PEP is converted to ATP and pyruvate. The generated pyruvate is quantified by colorimetric (λmax = 570 nm) or fluorometric methods (Ex/Em = 535/587 nm). The assay is simple, sensitive and reliable. The detection limit is approximately 1 µM PEP in biological samples.
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Platform
Microplate reader
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 100 tests PEP Assay Buffer 1 x 25ml PEP Converter 1 vial PEP Developer Mix 1 vial PEP Probe (in DMSO) 1 x 0.2ml PEP Standard (1 µmole) 1 vial -
Relevance
Phosphoenolpyruvate (PEP) is an important intermediate in carbohydrate metabolism. Containing a high-energy phosphate bond, PEP is involved in glycolysis and gluco-neogenesis and in the shikimate pathway as well as in carbon fixation in plants. Bacteria utilize PEP in the phosphor-transferase system to acquire sugars from the environment. In the glycolytic pathway, PEP is formed from 2-phosphoglycerate by enolase and generates ATP through the action of pyruvate kinase.
Images
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Phosphoenolpyruvic acid (PEP) Assay Kit (ab204713)
Typical PEP Standard calibration curve using colorimetric reading.
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Phosphoenolpyruvic acid (PEP) Assay Kit (ab204713)Typical PEP Standard calibration curve using fluorometric reading.