Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Integrin alpha 6 antibody [EPR5578] (ab124924)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR5578] to Integrin alpha 6
  • Suitable for: WB
  • Knockout validated
  • Reacts with: Human

Overview

  • Product name

    Anti-Integrin alpha 6 antibody [EPR5578]
    See all Integrin alpha 6 primary antibodies

  • Description

    Rabbit monoclonal [EPR5578] to Integrin alpha 6

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide within Human Integrin alpha 6 aa 100-200. The exact sequence is proprietary.
    Database link: P23229

  • Positive control

    • SW480, HEK293, JAR, HT1376, and Human platelet lysates; Human kidney tissue.

  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

Images

  • Western blot - Anti-Integrin alpha 6 antibody [EPR5578] (ab124924)
    Western blot - Anti-Integrin alpha 6 antibody [EPR5578] (ab124924)
    All lanes : Anti-Integrin alpha 6 antibody [EPR5578] (ab124924) at 1/1000 dilution

    Lane 1 : Wild-type SW480 cell lysate
    Lane 2 : ITGA6 knockout SW480 cell lysate
    Lane 3 : HEK-293 cell lysate

    Lysates/proteins at 20 µg per lane.

    Performed under reducing conditions.

    Predicted band size: 126 kDa
    Observed band size: 130 kDa
    why is the actual band size different from the predicted?



    Lanes 1 - 3: Merged signal (red and green). Green - ab124924 observed at 130 kDa. Red - loading control ab8245 (Mouse anti-GAPDH antibody [6C5]) observed at 37kDa.

    ab124924 was shown to react with Integrin alpha 6 in wild-type SW480 cells in western blot with loss of signal observed in ITGA6 knockout sample. Wild-type and ITGA6 knockout SW480 cell lysates were subjected to SDS-PAGE. Membranes were blocked in 3% milk in TBS-T (0.1% Tween®) before incubation with ab124924 and ab8245 (Mouse anti-GAPDH antibody [6C5]) overnight at 4°C at a 1 in 1000 dilution and a 1 in 20000 dilution respectively. Blots were incubated with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

  • Western blot - Anti-Integrin alpha 6 antibody [EPR5578] (ab124924)
    Western blot - Anti-Integrin alpha 6 antibody [EPR5578] (ab124924)
    All lanes : Anti-Integrin alpha 6 antibody [EPR5578] (ab124924) at 1/10000 dilution (purified)

    Lane 1 : HT-1376 cell lysate
    Lane 2 : JAR cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : HRP goat anti-rabbit IgG (H+L) at 1/50000 dilution

    Predicted band size: 126 kDa
    Observed band size: 126 kDa



    Blocking buffer: 5% NFDM/TBST
    Dilution buffer: 5% NFDM/TBST

  • Western blot - Anti-Integrin alpha 6 antibody [EPR5578] (ab124924)
    Western blot - Anti-Integrin alpha 6 antibody [EPR5578] (ab124924)
    All lanes : Anti-Integrin alpha 6 antibody [EPR5578] (ab124924) at 1/1000 dilution

    Lane 1 : JAR cell lysates
    Lane 2 : HT1376 cell lysates
    Lane 3 : Human platelet lysates

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : HRP-conjugated goat anti-rabbit at 1/2000 dilution

    Predicted band size: 126 kDa



    Secondary antibody - anti-rabbit HRP (ab6721)

  • OI-RD Scanning - Anti-Integrin alpha 6 antibody [EPR5578] (ab124924)
    OI-RD Scanning - Anti-Integrin alpha 6 antibody [EPR5578] (ab124924)
    Equilibrium disassociation constant (KD)
    Learn more about KD

    Click here to learn more about KD
  • Anti-Integrin alpha 6 antibody [EPR5578] (ab124924)
    Anti-Integrin alpha 6 antibody [EPR5578] (ab124924)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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