Anti-Integrin alpha 6 antibody [EPR18124] (ab181551)
![Anti-Integrin alpha 6 antibody [EPR18124] (ab181551)](https://www.abcam.com/ps/products/181/ab181551/Images/ab181551-449295-anti-integrin-alpha-6-antibody-epr18124-western-blot-sw480-wt-sw480-itga6-knockout-hek293.jpg "Anti-Integrin alpha 6 antibody [EPR18124] (ab181551)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR18124] to Integrin alpha 6
- Suitable for: WB, IHC-P
- Knockout validated
- Reacts with: Mouse, Rat, Human
Overview
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Product name
Anti-Integrin alpha 6 antibody [EPR18124]
See all Integrin alpha 6 primary antibodies -
Description
Rabbit monoclonal [EPR18124] to Integrin alpha 6 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IHC-P MouseRatHumanWB MouseRatHuman
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Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: SW480, A549, F6 whole cell lysates; Mouse skin lysate and Rat skin lysate, Human fetal kidney tissue lysate, Mouse kidney and spleen tissue lysate, Rat spleen tissue lysate. IHC-P: Human breast tissue, Human endometrial cancer tissue, Mouse stomach tissue, Rat stomach tissue.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR18124 -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-Integrin alpha 6 antibody [EPR18124] (ab181551)All lanes : Anti-Integrin alpha 6 antibody [EPR18124] (ab181551) at 1/2000 dilution
Lane 1 : Wild-type SW480 cell lysate
Lane 2 : ITGA6 knockout SW480 cell lysate
Lane 3 : HEK-293 cell lysate
Lysates/proteins at 20 µg per lane.
Performed under reducing conditions.
Predicted band size: 127 kDa
Observed band size: 130 kDa why is the actual band size different from the predicted?Lanes 1 - 3: Merged signal (red and green). Green - ab181551 observed at 130 kDa. Red - loading control ab8245 (Mouse anti-GAPDH antibody [6C5]) observed at 37kDa.
ab181551 was shown to react with Integrin alpha 6 in wild-type SW480 cells in western blot with loss of signal observed in ITGA6 knockout sample. Wild-type and ITGA6 knockout SW480 cell lysates were subjected to SDS-PAGE. Membranes were blocked in 3% milk in TBS-T (0.1% Tween®) before incubation with ab181551 and ab8245 (Mouse anti-GAPDH antibody [6C5]) overnight at 4°C at a 1 in 2000 dilution and a 1 in 20000 dilution respectively. Blots were incubated with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Integrin alpha 6 antibody [EPR18124] (ab181551)](https://www.abcam.com/ps/products/181/ab181551/Images/ab181551-245893-anti-integrin-alpha-6-antibody-epr18124-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Integrin alpha 6 antibody [EPR18124] (ab181551)Immunohistochemical analysis of paraffin-embedded Human breast tissue labeling Intergrin alpha 6 with ab181551 at 1/500 followed by Goat Anti-Rabbit IgG (HRP) at 1/500.
Membrane and cytoplasm staining on epithelial cells of Human breast tissue is observed.
Counter stained with Hematoxylin.
Negative control: Used PBS instead of primary antibody followed by Goat Anti-Rabbit IgG (HRP) at 1/500.Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Integrin alpha 6 antibody [EPR18124] (ab181551)](https://www.abcam.com/ps/products/181/ab181551/Images/ab181551-245894-anti-integrin-alpha-6-antibody-epr18124-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Integrin alpha 6 antibody [EPR18124] (ab181551)Immunohistochemical analysis of paraffin-embedded Human endometrial cancer tissue labeling Intergrin alpha 6 with ab181551 at 1/500 followed by Goat Anti-Rabbit IgG (HRP) at 1/500.
Membrane and cytoplasm staining on tumor cells of Human endometrial cancer tissue is observed.
Counter stained with Hematoxylin.
Negative control: Used PBS instead of primary antibody followed by Goat Anti-Rabbit IgG (HRP) at 1/500.Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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![Western blot - Anti-Integrin alpha 6 antibody [EPR18124] (ab181551)](https://www.abcam.com/ps/products/181/ab181551/Images/ab181551-245885-anti-integrin-alpha-6-antibody-epr18124-western-blot.jpg)
Western blot - Anti-Integrin alpha 6 antibody [EPR18124] (ab181551)All lanes : Anti-Integrin alpha 6 antibody [EPR18124] (ab181551) at 1/10000 dilution
Lane 1 : SW480 (Human colon adenocarcinoma cell line) whole cell lysate
Lane 2 : A549 (Human lung carcinoma) whole cell lysate
Lane 3 : Mouse skin lysate
Lane 4 : Rat skin lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution
Developed using the ECL technique.
Predicted band size: 127 kDa
Observed band size: 127 kDa
Exposure time: 3 minutesBlocking and diluting buffer was 5% NFDM /TBST.
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![Western blot - Anti-Integrin alpha 6 antibody [EPR18124] (ab181551)](https://www.abcam.com/ps/products/181/ab181551/Images/ab181551-245887-anti-integrin-alpha-6-antibody-epr18124-western-blot.jpg)
Western blot - Anti-Integrin alpha 6 antibody [EPR18124] (ab181551)Anti-Integrin alpha 6 antibody [EPR18124] (ab181551) at 1/2000 dilution + Human fetal kidney tissue lysate at 10 µg
Secondary
Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution
Developed using the ECL technique.
Predicted band size: 127 kDa
Observed band size: 127 kDa
Exposure time: 3 minutesBlocking and diluting buffer was 5% NFDM /TBST.
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![Western blot - Anti-Integrin alpha 6 antibody [EPR18124] (ab181551)](https://www.abcam.com/ps/products/181/ab181551/Images/ab181551-245888-anti-integrin-alpha-6-antibody-epr18124-western-blot.jpg)
Western blot - Anti-Integrin alpha 6 antibody [EPR18124] (ab181551)All lanes : Anti-Integrin alpha 6 antibody [EPR18124] (ab181551) at 1/2000 dilution
Lane 1 : Mouse kidney tissue lysate
Lane 2 : Mouse spleen tissue lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution
Developed using the ECL technique.
Predicted band size: 127 kDa
Observed band size: 127 kDa
Exposure time: 10 secondsBlocking and diluting buffer was 5% NFDM /TBST
-
![Western blot - Anti-Integrin alpha 6 antibody [EPR18124] (ab181551)](https://www.abcam.com/ps/products/181/ab181551/Images/ab181551-245889-anti-integrin-alpha-6-antibody-epr18124-western-blot.jpg)
Western blot - Anti-Integrin alpha 6 antibody [EPR18124] (ab181551)All lanes : Anti-Integrin alpha 6 antibody [EPR18124] (ab181551) at 1/10000 dilution
Lane 1 : F9 (mouse embryo testicular cancer cell line) whole cell lysate
Lane 2 : Rat spleen tissue lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution
Developed using the ECL technique.
Predicted band size: 127 kDa
Observed band size: 127 kDa
Exposure time: 3 minutesBlocking and diluting buffer was 5% NFDM /TBST
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Integrin alpha 6 antibody [EPR18124] (ab181551)](https://www.abcam.com/ps/products/181/ab181551/Images/ab181551-245895-anti-integrin-alpha-6-antibody-epr18124-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Integrin alpha 6 antibody [EPR18124] (ab181551)Immunohistochemical analysis of paraffin-embedded Mouse stomach tissue labeling Intergrin alpha 6 with ab181551 at 1/500 followed by Goat Anti-Rabbit IgG (HRP) at 1/500.
Basement membrane staining on tumor cells of Mouse stomach tissue is observed.
Counter stained with Hematoxylin.
Negative control: Used PBS instead of primary antibody followed by Goat Anti-Rabbit IgG (HRP) at 1/500.Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Integrin alpha 6 antibody [EPR18124] (ab181551)](https://www.abcam.com/ps/products/181/ab181551/Images/ab181551-245896-anti-integrin-alpha-6-antibody-epr18124-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Integrin alpha 6 antibody [EPR18124] (ab181551)Immunohistochemical analysis of paraffin-embedded Rat stomach tissue labeling Intergrin alpha 6 with ab181551 at 1/500 followed by Goat Anti-Rabbit IgG (HRP) at 1/500.
Basement membrane staining on tumor cells of Rat stomach tissue is observed.
Counter stained with Hematoxylin.
Negative control: Used PBS instead of primary antibody followed by Goat Anti-Rabbit IgG (HRP) at 1/500.Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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![Anti-Integrin alpha 6 antibody [EPR18124] (ab181551)](https://www.abcam.com/ps/products/181/ab181551/Images/ab181551-9-benefits-of-recombinant-antibodies.png)
Anti-Integrin alpha 6 antibody [EPR18124] (ab181551)
