Antibodies, Proteins, Kits and Reagents for Life Science

307 566 ₸ Product size

100 µl 307 566 ₸ 10 µl 90 460 ₸

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Anti-LAMC2 antibody [EPR23654-127] (ab274376)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR23654-127] to LAMC2
Suitable for: ICC, IP, Flow Cyt, WB
Reacts with: Human

Product name

Anti-LAMC2 antibody [EPR23654-127]
See all LAMC2 primary antibodies
Description

Rabbit monoclonal [EPR23654-127] to LAMC2
Host species

Rabbit

Tested Applications & Species

Application	Species
Flow Cyt	Human
ICC	Human
IP	Human
WB	Human

See all applications and species data

Immunogen

Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: A431 whole cell lysate. ICC: A431 cells. Flow cyt: A549 cells. IP: A431 whole cell lysate.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR23654-127
Isotype

IgG
Research areas

Immunocytochemistry - Anti-LAMC2 antibody [EPR23654-127] (ab274376)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized A431 cells labelling LAMC2 with ab274376 at 1/100 dilution, followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) antibody at 1/1000 dilution (Green). Confocal image showing cytoplasmic staining in A431 cell line. Low expression control: A549 (PMID: 25591736). ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor^® 594) was used to counterstain tubulin at 1/200 dilution (Red). The Nuclear counterstain was DAPI (Blue).

Secondary antibody only control: Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) at 1/1000 dilution.
Western blot - Anti-LAMC2 antibody [EPR23654-127] (ab274376)

All lanes : Anti-LAMC2 antibody [EPR23654-127] (ab274376) at 1/1000 dilution

Lane 1 : A431 (human epidermoid carcinoma epithelial cell), whole cell lysate at 20 µg
Lane 2 : A549 (human lung carcinoma epithelial cell), whole cell lysate
Lane 3 : WI-38 (human fetal lung fibroblast), whole cell lysate at 20 µg

Secondary
Lane 1 : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051))
Lane 2 : Goat Anti-Rabbit IgG H&L (HRP) (ab97051)
Lane 3 : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Predicted band size: 131 kDa
Observed band size: 140,80 kDa
why is the actual band size different from the predicted?

Blocking and diluting buffer and concentration: 5% NFDM/TBST.

Bands of 140KDa and 80KDa respectively represent the full length and endogenously cleaved form.

The expression profile observed is consistent with what has been described in the literature (PMID: 25591736, 21829200).

Low expression: A549 (PMID:25591736) and WI-38 (PMID: 10964684)

Expsure time: 3 minutes
Flow Cytometry - Anti-LAMC2 antibody [EPR23654-127] (ab274376)

Flow cytometric analysis of 4% paraformaldehyde fixed, 90% methanol permeabilized A549 (Human lung carcinoma epithelial cell)(Left) A431 (Human epidermoid carcinoma epithelial cell)(Right) cells labelling LAMC2 with ab274376 at 1/500 dilution (0.1ug) (Red) (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).

A Goat anti rabbit IgG (Alexa Fluor^® 488, ab150077) at 1/2000 dilution was used as the secondary antibody.

Low expression control: A549.
Immunoprecipitation - Anti-LAMC2 antibody [EPR23654-127] (ab274376)

LAMC2 was immunoprecipitated from 0.35 mg A431 (human epidermoid carcinoma epithelial cell), whole cell lysate with ab274376 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab274376 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP)(ab131366) was used at 1/5000 dilution.

Lane 1: A431 (human epidermoid carcinoma epithelial cell), whole cell lysate 10 ug

Lane 2: ab274376 IP in A431 whole cell lysate.

Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab274376 in A431 whole cell lysate.

Blocking and dilution buffer and concentration: 5% NFDM/TBST.

Exposure time: 41 seconds

Band of 140KDa represents the full length form, Bands of 100KDa and 80KDa represent the endogenously cleaved form.

The expression profile observed is consistent with what has been described in the literature (PMID: 21829200).
Anti-LAMC2 antibody [EPR23654-127] (ab274376)

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