Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-LAMC2 antibody [EPR23654-127] (ab274376)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR23654-127] to LAMC2
  • Suitable for: ICC, IP, Flow Cyt, WB
  • Reacts with: Human

Overview

  • Product name

    Anti-LAMC2 antibody [EPR23654-127]
    See all LAMC2 primary antibodies

  • Description

    Rabbit monoclonal [EPR23654-127] to LAMC2

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    ICC
    Human
    IP
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: A431 whole cell lysate. ICC: A431 cells. Flow cyt: A549 cells. IP: A431 whole cell lysate.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR23654-127

  • Isotype

    IgG

  • Research areas

Images

  • Immunocytochemistry - Anti-LAMC2 antibody [EPR23654-127] (ab274376)
    Immunocytochemistry - Anti-LAMC2 antibody [EPR23654-127] (ab274376)

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized A431 cells labelling LAMC2 with ab274376 at 1/100 dilution, followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution (Green). Confocal image showing cytoplasmic staining in A431 cell line. Low expression control: A549 (PMID: 25591736). ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200  dilution (Red). The Nuclear counterstain was DAPI (Blue).

    Secondary antibody only control: Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution.

  • Western blot - Anti-LAMC2 antibody [EPR23654-127] (ab274376)
    Western blot - Anti-LAMC2 antibody [EPR23654-127] (ab274376)
    All lanes : Anti-LAMC2 antibody [EPR23654-127] (ab274376) at 1/1000 dilution

    Lane 1 : A431 (human epidermoid carcinoma epithelial cell), whole cell lysate at 20 µg
    Lane 2 : A549 (human lung carcinoma epithelial cell), whole cell lysate
    Lane 3 : WI-38 (human fetal lung fibroblast), whole cell lysate at 20 µg

    Secondary
    Lane 1 : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051))
    Lane 2 : Goat Anti-Rabbit IgG H&L (HRP) (ab97051)
    Lane 3 : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 131 kDa
    Observed band size: 140,80 kDa
    why is the actual band size different from the predicted?



    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    Bands of 140KDa and 80KDa respectively represent  the full length and endogenously cleaved form.

    The expression profile observed is consistent with what has been described in the literature (PMID: 25591736, 21829200).

    Low expression: A549 (PMID:25591736) and WI-38 (PMID: 10964684)

    Expsure time: 3 minutes

     

  • Flow Cytometry - Anti-LAMC2 antibody [EPR23654-127] (ab274376)
    Flow Cytometry - Anti-LAMC2 antibody [EPR23654-127] (ab274376)

    Flow cytometric analysis of 4% paraformaldehyde fixed, 90% methanol permeabilized A549 (Human lung carcinoma epithelial cell)(Left) A431 (Human epidermoid carcinoma epithelial cell)(Right) cells labelling LAMC2 with ab274376 at 1/500 dilution (0.1ug) (Red) (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).

    A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody.

    Low expression control: A549.

  • Immunoprecipitation - Anti-LAMC2 antibody [EPR23654-127] (ab274376)
    Immunoprecipitation - Anti-LAMC2 antibody [EPR23654-127] (ab274376)

    LAMC2 was immunoprecipitated from 0.35 mg A431 (human epidermoid carcinoma epithelial cell), whole cell lysate with ab274376 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab274376 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP)(ab131366) was used at 1/5000 dilution.

    Lane 1: A431 (human epidermoid carcinoma epithelial cell), whole cell lysate 10 ug

    Lane 2: ab274376 IP in A431 whole cell lysate.

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab274376 in A431 whole cell lysate.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 41 seconds

    Band of 140KDa represents the full length form, Bands of 100KDa and 80KDa represent the endogenously cleaved form.

    The expression profile observed is consistent with what has been described in the literature (PMID: 21829200).

  • Anti-LAMC2 antibody [EPR23654-127] (ab274376)
    Anti-LAMC2 antibody [EPR23654-127] (ab274376)

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