Anti-53BP1 antibody [BL-250-1H11] (ab243868)
![Anti-53BP1 antibody [BL-250-1H11] (ab243868)](https://www.abcam.com/ps/products/243/ab243868/Images/ab243868-334261-anti-53bp1-antibody-bl-250-1h11-immunoprecipitation.jpg "Anti-53BP1 antibody [BL-250-1H11] (ab243868)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [BL-250-1H11] to 53BP1
- Suitable for: IP, WB
- Reacts with: Human
Overview
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Product name
Anti-53BP1 antibody [BL-250-1H11]
See all 53BP1 primary antibodies -
Description
Rabbit monoclonal [BL-250-1H11] to 53BP1 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IP HumanWB Human
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Immunogen
Synthetic peptide within Human 53BP1 aa 350-400. The exact sequence is proprietary. NP_005648.1 and Gene ID 7158.
Database link: Q12888 -
General notes
This product is sold under License from Bethyl Laboratories, Inc.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.8
Preservative: 0.09% Sodium azide
Constituents: 98% Borate buffered saline, 0.1% BSA -
Concentration information loading... -
Purification notes
Recombinant antibody was purified from cell culture supernatant. -
Clonality
Monoclonal -
Clone number
BL-250-1H11 -
Isotype
IgG -
Research areas
Images
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Immunoprecipitation - Anti-53BP1 antibody [BL-250-1H11] (ab243868)53BP1 was immunoprecipitated from 1 mg HEK-293T whole cell lysate with ab243868 at 20 µl per reaction Western blot was performed on the immunoprecipitate using ab243868 at 1/1000 dilution.
Lane 1: ab243868 IP in HEK-293T cell lysate.
Lane 2: Contol IgG in HEK-293T cell lysate.
Detection: Chemiluminescence with an exposure time of 10 seconds.
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![Western blot - Anti-53BP1 antibody [BL-250-1H11] (ab243868)](https://www.abcam.com/ps/products/243/ab243868/Images/ab243868-334260-anti-53bp1-antibody-bl-250-1h11-western-blot.jpg)
Western blot - Anti-53BP1 antibody [BL-250-1H11] (ab243868)Western blot analysis using ab243868 at 1/1000 dilution.
Lane 1: HeLa whole cell lysate (15 µg).
Lane 2: HEK-293T whole cell lysate (15 µg).
Lane3: MCF7 whole cell lysate (15 µg).
Lane 4: HepG2 whole cell lysate (15 µg).
Lane 5: A549 whole cell lysate (15 µg).
Lane 6: SW620 whole cell lysate (15 µg).
Lane 7: SK whole cell lysate (15 µg).
Lane 8: Jurkat whole cell lysate (15 µg).
A HRP-conjugated goat anti-rabbit IgG antibody was used as the secondary. Detection: chemiluminescence with an exposure time of 10 seconds
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![Anti-53BP1 antibody [BL-250-1H11] (ab243868)](https://www.abcam.com/ps/products/243/ab243868/Images/ab243868-3-benefits-of-recombinant-antibodies.png)
Anti-53BP1 antibody [BL-250-1H11] (ab243868)
