RPA14/RPA3 overexpression 293T lysate (whole cell) (ab94054)
Overview
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Product name
RPA14/RPA3 overexpression 293T lysate (whole cell) -
General notes
ab94054 is a 293T cell transfected lysate in which Human RPA14/RPA3 has been transiently over-expressed using a pCMV-RPA14/RPA3 plasmid. The lysate is provided in 1X Sample Buffer. Note: For more details about how the transfected lysate was prepared view preparation notes
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Tested applications
Suitable for: WBmore details
Properties
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Mycoplasma free
Yes -
Form
Liquid -
Storage instructions
Shipped on dry ice. Upon delivery aliquot and store at -20ºC. Avoid freeze / thaw cycles. -
Storage buffer
Constituents: 0.01% Bromophenol blue, 2.3% Beta mercaptoethanol, 2% Sodium lauryl sulfate, 0.788% Tris HCl, 10% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Research areas
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Background
Function: Required for DNA recombination, repair and replication. The activity of RP-A is mediated by single-stranded DNA binding and protein interactions. Functions as component of the alternative replication protein A complex (aRPA). aRPA binds single-stranded DNA and probably plays a role in DNA repair; it does not support chromosomal DNA replication and cell cycle progression through S-phase. In vitro, aRPA cannot promote efficient priming by DNA polymerase alpha but supports DNA polymerase delta synthesis in the presence of PCNA and replication factor C (RFC), the dual incision/excision reaction of nucleotide excision repair and RAD51-dependent strand exchange.
Images
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SDS-PAGE - RPA14/RPA3 overexpression 293T lysate (whole cell) (ab94054)ab94054 at 15 µg/lane on an SDS-PAGE gel.
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Western blot - RPA14/RPA3 overexpression 293T lysate (whole cell) (ab94054)All lanes : Anti-RPA14/RPA3 antibody (ab58317) at 1/500 dilution
Lane 1 :RPA14/RPA3 overexpression 293T lysate (whole cell) (ab94054)
Lane 2 : 293T non-transfected Lysate
Lysates/proteins at 25 µg per lane.
Secondary
All lanes : Goat Anti-mouse IgG (H and L) HRP conjugated at 1/2500 dilution