Anti-BRCC36 antibody [EPR4365] (ab108295)
![Anti-BRCC36 antibody [EPR4365] (ab108295)](https://www.abcam.com/ps/products/108/ab108295/Images/ab108295-280816-anti-brcc36-antibody-epr4365-western-blot.jpg "Anti-BRCC36 antibody [EPR4365] (ab108295)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR4365] to BRCC36
- Suitable for: WB
- Knockout validated
- Reacts with: Human
Overview
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Product name
Anti-BRCC36 antibody [EPR4365]
See all BRCC36 primary antibodies -
Description
Rabbit monoclonal [EPR4365] to BRCC36 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB Human
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Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- HAP1, MCF7, SKBR3, 293T, Human fetal kidney and HeLa whole cell lysate (ab150035).
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 0.31% Sodium citrate, 0.175% Sodium chloride, 0.0172% EDTA, 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR4365 -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-BRCC36 antibody [EPR4365] (ab108295)
Lane 1: Wild type HAP1 whole cell lysate (40 µg)
Lane 2: Empty Lane
Lane 3: BRCC36 knockout HAP1 whole cell lysate (40 µg)
Lane 4: MCF7 whole cell lysate (40 µg)Lanes 1 - 4: Merged signal (red and green). Green - ab108295 observed at 36 kDa. Red - loading control, ab18058, observed at 130 kDa.
ab108295 was shown to recognize empty when empty knockout samples were used, along with additional cross-reactive bands. Wild-type and empty knockout samples were subjected to SDS-PAGE. Ab108295 and ab18058 (Mouse anti Vinculin loading control) were incubated overnight at 4°C at 1000 dilution and 1/10000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.
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![Western blot - Anti-BRCC36 antibody [EPR4365] (ab108295)](https://www.abcam.com/ps/products/108/ab108295/Images/ab108295-120803-anti-brcc36-antibody-epr4365-western-blot.jpg)
Western blot - Anti-BRCC36 antibody [EPR4365] (ab108295)All lanes : Anti-BRCC36 antibody [EPR4365] (ab108295) at 1/1000 dilution
Lane 1 : MCF7 cell lysate
Lane 2 : SKBR3 cell lysate
Lane 3 : 293T cell lysate
Lane 4 : Human fetal kidney lysate
Lane 5 : HeLa cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 36 kDa
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![Anti-BRCC36 antibody [EPR4365] (ab108295)](https://www.abcam.com/ps/products/108/ab108295/Images/ab108295-3-benefits-of-recombinant-antibodies.png)
Anti-BRCC36 antibody [EPR4365] (ab108295)
