Anti-BRCC36 antibody (ab115172)
Key features and details
- Rabbit polyclonal to BRCC36
- Suitable for: WB, IP, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-BRCC36 antibody
See all BRCC36 primary antibodies -
Description
Rabbit polyclonal to BRCC36 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IP, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Horse, Pig, Rhesus monkey, Orangutan, Bat, Elephant
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Immunogen
Synthetic peptide, corresponding to a region within amino acids 266 -316 of Human BRCC36 (P46736).
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Positive control
- HeLa, 293T and mouse NIH3T3 cell lysates.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7
Preservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate
pH 7 to 8. -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-BRCC36 antibody (ab115172)All lanes : Anti-BRCC36 antibody (ab115172) at 0.1 µg/ml
Lane 1 : HeLa cell lysate at 50 µg
Lane 2 : HeLa cell lysate at 15 µg
Lane 3 : HeLa cell lysate at 5 µg
Lane 4 : 293T cell lysate at 50 µg
Lane 5 : Mouse NIH3T3 cell lysate at 50 µg
Predicted band size: 36 kDa
Exposure time: 30 seconds
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BRCC36 antibody (ab115172)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human prostate carcinoma tissue labelling BRCC36 with ab115172 at 1/1000 (1µg/ml). Detection: DAB. -
Immunoprecipitation - Anti-BRCC36 antibody (ab115172)Immunoprecipitation of BRCC36 (3 µg of antibody per mg of HeLa cell lysate; 20% of the IP loaded). Lane 1-Immunoprecipitation with an anti-BRCC36 antibody that recognizes an upstream epitope; Lane 2-Immunoprecipitation with anti-BRCC36 antibody (ab115172); Lane 3-Immunoprecipitation with IgG control. Anti-BRCC36 antibody (ab115172) used at 0.4 µg/ml for western blot. Detection: Chemiluminescence with exposure time of 30 sec.
