Recombinant human Acetyl Coenzyme A Carboxylase beta protein (Active) (ab271358)
Key features and details
- Expression system: Baculovirus infected Sf9 cells
- Purity: >= 80% SDS-PAGE
- Active: Yes
- Tags: His tag C-Terminus
- Suitable for: Functional Studies, SDS-PAGE
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Product name
Recombinant human Acetyl Coenzyme A Carboxylase beta protein (Active) -
Biological activity
Specific Activity: ≥20 pmoles/min/µgAssay Conditions: 25 μl Acetyl Coenzyme A Carboxylase beta reaction is conducted in a buffer containing 30 mM HEPES (pH 7.4), 2 mM MgCl2, 2 mM potassium citrate, 1 mM DTT, 100 μM ATP, 12 mM NaHCO3 and 20 μM acetyl-CoA at 30°C for 45 min. ATP reduction is detected. -
Purity
>= 80 % SDS-PAGE. -
Expression system
Baculovirus infected Sf9 cells -
Accession
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Protein length
Full length protein -
Animal free
No -
Nature
Recombinant -
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Species
Human -
Predicted molecular weight
277 kDa including tags -
Tags
His tag C-Terminus
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Preparation and Storage
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Stability and Storage
Shipped on Dry Ice. Store at -80°C. Avoid freeze / thaw cycle.
pH: 8.00
Preservative: 0.68% Imidazole
Constituents: 0.63% Tris HCl, 0.64% Sodium chloride, 0.02% Potassium chloride, 0.04% Tween, 0.05% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 20% Glycerol (glycerin, glycerine)This product is an active protein and may elicit a biological response in vivo, handle with caution.
Images
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Functional Studies - Recombinant human Acetyl Coenzyme A Carboxylase beta protein (Active) (ab271358)Specific activity of ab271358.
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SDS-PAGE - Recombinant human Acetyl Coenzyme A Carboxylase beta protein (Active) (ab271358)SDS-PAGE analysis of ab271358.