Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Cdc6 (phospho S54) antibody [EPR759Y] - BSA and Azide free (ab247402)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR759Y] to Cdc6 (phospho S54) - BSA and Azide free
  • Suitable for: ICC/IF, WB, Dot blot
  • Reacts with: Human

Overview

  • Product name

    Anti-Cdc6 (phospho S54) antibody [EPR759Y] - BSA and Azide free
    See all Cdc6 primary antibodies

  • Description

    Rabbit monoclonal [EPR759Y] to Cdc6 (phospho S54) - BSA and Azide free

  • Host species

    Rabbit

  • Specificity

    This antibody recognises Cdc6 only when phosphorylated at Serine 54. 

  • Tested Applications & Species

    Application Species
    ICC/IF
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Raji + FBS cell lysates. ICC: HeLa cells. Dot Blot: Cdc6 (pS54) phospho peptide.

  • General notes

    ab247402 is the carrier-free version of ab75809 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    Ab247402 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-Cdc6 (phospho S54) antibody [EPR759Y] - BSA and Azide free (ab247402)
    Western blot - Anti-Cdc6 (phospho S54) antibody [EPR759Y] - BSA and Azide free (ab247402)
    All lanes : Anti-Cdc6 (phospho S54) antibody [EPR759Y] (ab75809) at 1/5000 dilution

    Lane 1 : Hela (human cervix adenocarcinoma epithelial cell) whole cell lysate
    Lane 2 : Hela (human cervix adenocarcinoma epithelial cell) treated with Hydroxyurea (3mM, 20 h) whole cell lysate
    Lane 3 : Hela (human cervix adenocarcinoma epithelial cell) treated with Hydroxyurea (3mM, 20 h) whole cell lysate. Then the membrane was incubated with phosphatase.

    Lysates/proteins at 15 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 63 kDa


    Exposure time: 30 seconds


    Blocking buffer and diluting buffer: 5% NFDM/TBST

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab75809).

  • Dot Blot - Anti-Cdc6 (phospho S54) antibody [EPR759Y] - BSA and Azide free (ab247402)
    Dot Blot - Anti-Cdc6 (phospho S54) antibody [EPR759Y] - BSA and Azide free (ab247402)

    This data was developed using the same antibody clone in a different buffer formulation (ab97051).

    Primary antibody dilution 1:1000 dilution. Secondary antibody used was Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051). Secondary ab dilution 1:100,000 dilution. Blocking buffer and concentration 5% NFDM/TBST. Diluting buffer and concentration 5% NFDM /TBST. Lane 1:Cdc6 (pS54) phospho peptide, Lane 2:Cdc6 non-phospho peptide. Exposure time 3 minutes

  • Immunocytochemistry/ Immunofluorescence - Anti-Cdc6 (phospho S54) antibody [EPR759Y] - BSA and Azide free (ab247402)
    Immunocytochemistry/ Immunofluorescence - Anti-Cdc6 (phospho S54) antibody [EPR759Y] - BSA and Azide free (ab247402)

    Cell line 293T (Human embryonic kidney epithelial cell). Target AbID Ab75809 anti- Cdc6 (phospho S54).

    ab150077 AlexaFluor®488 Goat anti-Rabbit secondary. Counterstain AbID Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594). Fixative 4% Paraformaldehyde. Permeabilisation 0.1% tritonX-100. Nuclear counter stain DAPI. Datasheet Comments Confocal image showing the expression was increased after treatment with Hydroxyurea (3mM 20hour), the signal was decreased after treatment with the Lambda Protein Phosphatase 31℃ for 2h. Target 1oAb dilution 1:100 2.4 μg/ml, Target 2ndry Ab dilution 1:1000 2 μg/mL, Counterstain Ab dilution 1:200 2.5 μg/mL.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab75809).

  • Anti-Cdc6 (phospho S54) antibody [EPR759Y] - BSA and Azide free (ab247402)
    Anti-Cdc6 (phospho S54) antibody [EPR759Y] - BSA and Azide free (ab247402)

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