DHEA sulfate (DHEA-S) ELISA Kit (ab108669)
Key features and details
- Sensitivity: 0.04 ng/ml
- Range: 0.1 µg/ml - 10 µg/ml
- Sample type: Cit plasma, EDTA Plasma, Hep Plasma, Serum
- Detection method: Colorimetric
- Assay type: Competitive
- Reacts with: Human
Overview
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Product name
DHEA sulfate (DHEA-S) ELISA Kit
See all DHEA-S kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Control 64 Inter-assay Sample n Mean SD CV% Control 48 -
Sample type
Serum, Hep Plasma, EDTA Plasma, Cit plasma -
Assay type
Competitive -
Sensitivity
= 0.04 ng/ml -
Range
0.1 µg/ml - 10 µg/ml -
Recovery
= 96.71 %
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Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Human -
Product overview
Abcam’s DHEA sulfate (DHEA-S) in vitro competitive ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate quantitative measurement of DHEA sulfate in plasma and serum.
A 96-well plate has been precoated with anti-DHEA sulphate antibodies. Samples and the DHEA sulfate-HRP conjugate are added to the wells, where DHEA sulfate in the sample competes with the added DHEA sulfate-HRP for antibody binding. After incubation, the wells are washed to remove unbound material and TMB substrate is then added which is catalyzed by HRP to produce blue coloration. The reaction is terminated by addition of Stop Solution which stops the color development and produces a color change from blue to yellow. The intensity of signal is inversely proportional to the amount of DHEA sulfate in the sample and the intensity is measured at 450 nm.
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Platform
Microplate
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 10X Wash Solution 1 x 50ml Serum Diluent 1 x 100ml Anti-DHEA sulfate antibody Coated Microplate (12 x 8 wells) 1 unit Control A 1 x 1ml Control B 1 x 1ml Cover foils 1 unit DHEA sulfate Standard 0 – 0 µg/mL 1 x 1ml DHEA sulfate Standard 1 – 0.1 µg/mL 1 x 1ml DHEA sulfate Standard 2 – 0.4 µg/mL 1 x 1ml DHEA sulfate Standard 3 – 1.0 µg/mL 1 x 1ml DHEA sulfate Standard 4 – 4.0 µg/mL 1 x 1ml DHEA sulfate Standard 5 – 10.0 µg/mL 1 x 1ml DHEA sulfate-HRP Conjugate 1 x 12ml Stop Solution 1 x 15ml Strip holder 1 unit TMB Substrate Solution 1 x 15ml -
Research areas
Images
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Typical Standard Curve
Representative Standard Curve using ab108669
