Human Estradiol E2 ELISA Kit (ab108640)
Key features and details
- Sensitivity: 10 pg/ml
- Range: 10 pg/ml - 1000 pg/ml
- Sample type: Serum
- Detection method: Colorimetric
- Assay type: Competitive
- Reacts with: Human
Overview
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Product name
Human Estradiol E2 ELISA Kit -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% 13 pg/mL 24 3 24.1% 73 pg/mL 24 7 10.3% 247 pg/mL 24 10 4.1% 633 pg/mL 24 31 4.9% Inter-assay Sample n Mean SD CV% 14 pg/mL 20 4 26.7% 82 pg/mL 20 8 10.3% 264 pg/mL 20 17 6.4% 691 pg/mL 20 45 6.6% -
Sample type
Serum -
Assay type
Competitive -
Sensitivity
10 pg/ml -
Range
10 pg/ml - 1000 pg/ml -
Recovery
101.3 %
Sample specific recovery Sample type Average % Range Serum 101.3 18pg/ml - 810pg/ml -
Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Human -
Product overview
Abcam’s Estradiol E2 in vitro competitive ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate quantitative measurement of Estradiol E2 in Human serum.
A 96-well plate has been pre-coated with goat anti-rabbit IgG. Samples, standards and the rabbit anti-Estradiol E2 Reagent and Estradiol E2-HRP conjugate are added to the wells, where Estradiol E2 in the sample competes with the added Estradiol E2-HRP for antibody binding. After incubation, the wells are washed to remove unbound material and TMB substrate is then added which is catalyzed by HRP to produce blue coloration. The reaction is terminated by addition of Stop Solution which stops the color development and produces a color change from blue to yellow. The intensity of signal is inversely proportional to the amount of Estradiol E2 in the sample and the intensity is measured at 450 nm.
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Platform
Microplate
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components Identifier 1 x 96 tests Estradiol E2 Control 1 1 x 0.5ml Estradiol E2 Control 2 1 x 0.5ml Estradiol E2 Standard 1 - 0 pg/mL 1 x 0.5ml Estradiol E2 Standard 2 - 10 pg/mL 1 x 0.5ml Estradiol E2 Standard 3 - 30 pg/mL 1 x 0.5ml Estradiol E2 Standard 4 - 100 pg/mL 1 x 0.5ml Estradiol E2 Standard 5 - 300 pg/mL 1 x 0.5ml Estradiol E2 Standard 6 - 1000 pg/mL 1 x 0.5ml Estradiol E2-HRP Conjugate Reagent Blue colour 1 x 12ml Goat anti-rabbit IgG Coated Microplate (12 x 8 wells) 1 x 96 tests Rabbit Anti-Estradiol E2 Reagent Pink colour 1 x 7ml Stop Solution 1 x 11ml TMB Reagent 1 x 11ml -
Research areas
Images
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Typical Standard Curve
Representative standard curve using ab108640
