Anti-NDUFB10 antibody [EPR16230] (ab192245)
![Anti-NDUFB10 antibody [EPR16230] (ab192245)](https://www.abcam.com/ps/products/192/ab192245/Images/ab192245-229910-anti-ndufb10-antibody-epr16230-western-blot.jpg "Anti-NDUFB10 antibody [EPR16230] (ab192245)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR16230] to NDUFB10
- Suitable for: WB, ICC/IF
- Reacts with: Human
Overview
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Product name
Anti-NDUFB10 antibody [EPR16230]
See all NDUFB10 primary antibodies -
Description
Rabbit monoclonal [EPR16230] to NDUFB10 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species ICC/IF HumanWB Human
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Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- HeLa, HepG2, Jurkat and Human fetal kidney lysates; HeLa cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 0.05% BSA, 40% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR16230 -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-NDUFB10 antibody [EPR16230] (ab192245)All lanes : Anti-NDUFB10 antibody [EPR16230] (ab192245) at 1/5000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : HepG2 cell lysate
Lane 3 : Jurkat cell lysate
Lane 4 : Human fetal kidney lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 21 kDa
Observed band size: 21 kDaBlocking/Dilution buffer: 5% NFDM /TBST.
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![Immunocytochemistry/ Immunofluorescence - Anti-NDUFB10 antibody [EPR16230] (ab192245)](https://www.abcam.com/ps/products/192/ab192245/Images/ab192245-229908-anti-ndufb10-antibody-epr16230-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-NDUFB10 antibody [EPR16230] (ab192245)Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% tritonX-100 permeabilized HeLa cells labeling NDUFB10 with ab192245 at 1/50 dilution ( 6 μg/ml), followed by Goat anti rabbit IgG (Alexa Fluor® 555) secondary antibody (ab150078) at 1/500 dilution. Nuclear counter stain Dapi (blue).
The two negative controls are ab192245 at 1/50 dilution followed by (Goat anti mouse IgG (Alexa Fluor®488) secondary antibody at 1/200 dilution.
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![Anti-NDUFB10 antibody [EPR16230] (ab192245)](https://www.abcam.com/ps/products/192/ab192245/Images/ab192245-3-benefits-of-recombinant-antibodies.png)
Anti-NDUFB10 antibody [EPR16230] (ab192245)
