Antibodies, Proteins, Kits and Reagents for Life Science

284 784 ₸ Product size

100 µl 284 784 ₸

Order now and get it on Wednesday March 10, 2021

Anti-NDUFAF4 antibody [EPR15931] (ab191414)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR15931] to NDUFAF4
Suitable for: WB, IP, ICC/IF
Reacts with: Human

Product name

Anti-NDUFAF4 antibody [EPR15931]
See all NDUFAF4 primary antibodies
Description

Rabbit monoclonal [EPR15931] to NDUFAF4
Host species

Rabbit

Tested Applications & Species

Application	Species
ICC/IF	Human
IP	Human
WB	Human

See all applications and species data

Immunogen

Recombinant full length protein. This information is proprietary to Abcam and/or its suppliers.
Positive control
- T47D, Raji, K562, MCF7 and MDA-MB-435 cell lysates; HeLa cells
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR15931
Isotype

IgG
Research areas

Immunocytochemistry/ Immunofluorescence - Anti-NDUFAF4 antibody [EPR15931] (ab191414)

Immunofluorescence analysis of HeLa cells labeling NDUFAF4 using ab191414 at 1/150 dilution (9.5 μg/mL). A Goat anti Rabbit IgG (Alexa Fluor488) at 1/200 dilution (ab150077) was used as secondary antibody. Cells were fixed with 4% Paraformaldehyde and permealized with 0.1% triton X-100. Counterstain: DAPI. The 2 negative cotrols images were obtained using Goat anti Mouse IgG (Alexa Fluor®594) at 1:400 dilution as secondary antibody.
Western blot - Anti-NDUFAF4 antibody [EPR15931] (ab191414)

All lanes : Anti-NDUFAF4 antibody [EPR15931] (ab191414) at 1/10000 dilution

Lane 1 : T47D cell lysate
Lane 2 : Raji cell lysate
Lane 3 : K562 cell lysate
Lane 4 : MDA-MB-435 cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 20 kDa
Western blot - Anti-NDUFAF4 antibody [EPR15931] (ab191414)

Anti-NDUFAF4 antibody [EPR15931] (ab191414) at 1/1000 dilution + MCF7 cell lysate at 10 µg

Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 20 kDa
Immunoprecipitation - Anti-NDUFAF4 antibody [EPR15931] (ab191414)

Immunoprecipitation analysis of MDA-MB-435 cell lysate labeling NDUFAF4 using ab191414 at 1/90 dilution (Lane 1). An Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1500 was used as secondary antibody. Lane 2: PBS instead of MDA-MB-435 cell lysate.
Anti-NDUFAF4 antibody [EPR15931] (ab191414)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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