Anti-FH/Fumarase antibody [8F12BB5] (ab110286)
![Anti-FH/Fumarase antibody [8F12BB5] (ab110286)](https://www.abcam.com/ps/products/110/ab110286/Images/FH-Primary-antibodies-ab110286-5.jpg "Anti-FH/Fumarase antibody [8F12BB5] (ab110286)")
Key features and details
- Mouse monoclonal [8F12BB5] to FH/Fumarase
- Suitable for: IP, IHC-P, ICC/IF, Flow Cyt
- Reacts with: Rat, Cow, Human
- Isotype: IgG2a
Overview
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Product name
Anti-FH/Fumarase antibody [8F12BB5]
See all FH/Fumarase primary antibodies -
Description
Mouse monoclonal [8F12BB5] to FH/Fumarase -
Host species
Mouse -
Tested Applications & Species
See all applications and species dataApplication Species Flow Cyt HumanICC/IF HumanIHC-P HumanIP RatCowHuman
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Immunogen
Full length native protein (purified). This information is considered to be commercially sensitive.
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Positive control
- Human HDFn cells; Human cerebellum tissue; HepG2 cells, human liver mitochondria, bovine liver mitochondria, and rat liver mitochondria; HeLa cells
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General notes
This antibody clone is manufactured by Abcam.
Product was previously marketed under the MitoSciences sub-brand.
If you require this antibody in a particular buffer formulation or a particular conjugate for your experiments, please contact orders@abcam.com or you can find further information here.
This product was previously labelled as FH
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
Preservative: 0.02% Sodium azide
Constituent: HEPES buffered saline -
Concentration information loading... -
Purity
Ammonium Sulphate Precipitation -
Purification notes
Purity near homogeneity as judged by SDS-PAGE. The antibody was produced in-vitro using hybridomas grown in serum-free medium and then purified by biochemical fractionation. -
Clonality
Monoclonal -
Clone number
8F12BB5 -
Isotype
IgG2a -
Light chain type
kappa -
Research areas
Images
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Flow Cytometry - Anti-FH/Fumarase antibody [8F12BB5] (ab110286)HeLa cells were stained with 1 µg/mL ab110286 (blue) or an equal amount of an isotype control antibody (red) and analyzed by flow cytometry.
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![Immunocytochemistry/ Immunofluorescence - Anti-FH/Fumarase antibody [8F12BB5] (ab110286)](https://www.abcam.com/ps/products/110/ab110286/Images/FH-Primary-antibodies-ab110286-2.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-FH/Fumarase antibody [8F12BB5] (ab110286)Immunocytochemistry image of FH/Fumarase (ab110286) stained human HDFn cells. The cells were paraformaldehyde fixed (4%, 20 min) and Triton X-100 permeabilized (0.1%, 15min). The cells were incubated with ab110286 at 5µg/ml for 2h at room temperature or over night at 4°C. The secondary antibody was (red) Alexa Fluor® 594 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. 10% Goat serum was used as the blocking agent for all blocking steps. DAPI was used to stain the cell nuclei (blue). Target protein locates mainly in mitochondria.
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![Immunoprecipitation - Anti-FH/Fumarase antibody [8F12BB5] (ab110286)](https://www.abcam.com/ps/products/110/ab110286/Images/FH-Primary-antibodies-ab110286-3.jpg)
Immunoprecipitation - Anti-FH/Fumarase antibody [8F12BB5] (ab110286)FH/Fumarase immunocaptured from HepG2 cells (Lane 1), human liver mitochondria (Lane 2), bovine liver mitochondria (Lane 3), and rat liver mitochondria (Lane 4) using ab110286.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FH/Fumarase antibody [8F12BB5] (ab110286)](https://www.abcam.com/ps/products/110/ab110286/Images/FH-Primary-antibodies-ab110286-4.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FH/Fumarase antibody [8F12BB5] (ab110286)ab110286 staining FH/Fumarase in formalin-fixed, paraffin-embedded human cerebellum tissue by Immunohistochemistry at 5ug/ml. FH/Fumarase immunoactivity is most intense in neuronal cell bodies, most notably in the large Purkinje cells.
