Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Aldolase antibody [EPR23181-39] - BSA and Azide free (ab273636)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR23181-39] to Aldolase - BSA and Azide free
  • Suitable for: IHC-P, WB, ICC, Flow Cyt
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-Aldolase antibody [EPR23181-39] - BSA and Azide free
    See all Aldolase primary antibodies

  • Description

    Rabbit monoclonal [EPR23181-39] to Aldolase - BSA and Azide free

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    ICC
    Mouse
    IHC-P
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: MCF-7, HepG2, SH-SY5Y,THP-1, C2C12, C6, A549 and NIH/3T3 whole cell lysates; human brain, colon, colon cancer and skeletal muscle tissue lysates; Mouse brain and skeletal muscle tissue lysates; rat brain and skeletal muscle tissue lysates. IHC-P: Human lung cancer and colon cancer tissue; Mouse skeletal muscle tissue; Rat skeletal muscle tissue. ICC: A549 and NIH/3T3 cells. Flow cyt: A549 and NIH/3T3 cells.

  • General notes

    ab273636 is the carrier-free version of ab252953. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

Images

  • Immunocytochemistry - Anti-Aldolase antibody [EPR23181-39] - BSA and Azide free (ab273636)
    Immunocytochemistry - Anti-Aldolase antibody [EPR23181-39] - BSA and Azide free (ab273636)

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized A549 cells labelling Aldolase with ab252953 at 1/50 dilution, followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) antibody at 1/1000 (2 ug/ml) dilution (Green). Confocal image showing strong cytoplasmic and weak nuclear staining in A549 cells. ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 dilution (Red). The Nuclear counterstain was DAPI (Blue).

    Secondary antibody only control: Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 (2 ug/ml) dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab252953).

  • Western blot - Anti-Aldolase antibody [EPR23181-39] - BSA and Azide free (ab273636)
    Western blot - Anti-Aldolase antibody [EPR23181-39] - BSA and Azide free (ab273636)
    All lanes : Anti-Aldolase antibody [EPR23181-39] (ab252953) at 1/1000 dilution

    Lane 1 : MCF7 (human breast adenocarcinoma epithelial cell) whole cell lysate at 40 µg
    Lane 2 : HepG2 (human hepatocellular carcinoma epithelial cell) whole cell lysate at 40 µg
    Lane 3 : SH-SY5Y (human neuroblastoma epithelial cell) whole cell lysate at 40 µg
    Lane 6 : C6 (rat glial tumor glial cell) whole cell lysate at 40 µg
    Lane 7 : A549 (human lu carcinoma epithelial cell) whole cell lysate
    Lane 8 : NIH/3T3 (mouse embryonic fibroblast) whole cell lysate at 40 µg

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/50000 dilution

    Predicted band size: 39 kDa
    Observed band size: 39 kDa



    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    Exposure times: Lanes 1-6: 5.5 seconds; Lanes 7-8: 26 seconds.  

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab252953).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aldolase antibody [EPR23181-39] - BSA and Azide free (ab273636)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aldolase antibody [EPR23181-39] - BSA and Azide free (ab273636)

    Immunohistochemical analysis of paraffin-embedded Rat skeletal muscle tissue labeling Aldolase with ab252953 at 1/50 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining in rat skeletal muscle (PMID: 18676612). The section was incubated with ab252953 for 20 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab252953).

  • Flow Cytometry - Anti-Aldolase antibody [EPR23181-39] - BSA and Azide free (ab273636)
    Flow Cytometry - Anti-Aldolase antibody [EPR23181-39] - BSA and Azide free (ab273636)

    Flow cytometric analysis of 4% paraformaldehyde fixed, 90% methanol permeabilized A549 (Human lung carcinoma epithelial cell) cells labelling Aldolase with ab252953 at 1/500 dilution (0.1ug) (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab252953).

  • Western blot - Anti-Aldolase antibody [EPR23181-39] - BSA and Azide free (ab273636)
    Western blot - Anti-Aldolase antibody [EPR23181-39] - BSA and Azide free (ab273636)
    All lanes : Anti-Aldolase antibody [EPR23181-39] (ab252953) at 1/1000 dilution

    Lane 1 : Rat brain tissue lysate
    Lane 2 : Rat skeletal muscle tissue lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/100000 dilution

    Predicted band size: 39 kDa



    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    Exposure times: Lane 1: 10 seconds; Lane 2: 3 seconds. 

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab252953).

  • Flow Cytometry - Anti-Aldolase antibody [EPR23181-39] - BSA and Azide free (ab273636)
    Flow Cytometry - Anti-Aldolase antibody [EPR23181-39] - BSA and Azide free (ab273636)

    Flow cytometric analysis of 4% paraformaldehyde fixed, 90% methanol permeabilized NIH/3T3 (Mouse embryonic fibroblast) cells labelling Aldolase with ab252953 at 1/50 dilution (1ug) (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab252953).

  • Immunocytochemistry - Anti-Aldolase antibody [EPR23181-39] - BSA and Azide free (ab273636)
    Immunocytochemistry - Anti-Aldolase antibody [EPR23181-39] - BSA and Azide free (ab273636)

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized NIH/3T3 cells labelling Aldolase with ab252953 at 1/50 dilution, followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) antibody at 1/1000 (2 ug/ml) dilution (Green). Confocal image showing strong cytoplasmic and weak nuclear staining in NIH/3T3 cells. ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 dilution (Red). The Nuclear counterstain was DAPI (Blue).

    Secondary antibody only control: Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 (2 ug/ml) dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab252953).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aldolase antibody [EPR23181-39] - BSA and Azide free (ab273636)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aldolase antibody [EPR23181-39] - BSA and Azide free (ab273636)

    Immunohistochemical analysis of paraffin-embedded Mouse skeletal muscle tissue labeling Aldolase with ab252953 at 1/50 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining in mouse skeletal muscle (PMID: 18676612). The section was incubated with ab252953 for 20 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument.  Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab252953).

  • Western blot - Anti-Aldolase antibody [EPR23181-39] - BSA and Azide free (ab273636)
    Western blot - Anti-Aldolase antibody [EPR23181-39] - BSA and Azide free (ab273636)
    All lanes : Anti-Aldolase antibody [EPR23181-39] (ab252953) at 1/1000 dilution

    Lane 1 : Human brain tissue lysate
    Lane 2 : Human skeletal muscle tissue lysate
    Lane 3 : Mouse brain tissue lysate
    Lane 4 : Mouse skeletal muscle tissue lysate
    Lane 5 : Human colon cancer tissue lysate

    Lysates/proteins at 40 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/50000 dilution

    Predicted band size: 39 kDa
    Observed band size: 39 kDa



    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    Exposure times: Lanes 1-3: 3 seconds; Lane 4-5: 5.5 seconds.  

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab252953).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aldolase antibody [EPR23181-39] - BSA and Azide free (ab273636)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aldolase antibody [EPR23181-39] - BSA and Azide free (ab273636)

    Immunohistochemical analysis of paraffin-embedded Human colon cancer tissue labeling Aldolase with ab252953 at 1/100 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining in human colon cancer (PMID: 29453983). The section was incubated with ab252953 for 20 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab252953).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aldolase antibody [EPR23181-39] - BSA and Azide free (ab273636)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aldolase antibody [EPR23181-39] - BSA and Azide free (ab273636)

    Immunohistochemical analysis of paraffin-embedded Human lung cancer (A) and its adjacent noncancerous tissue (B) tissue labeling Aldolase with ab252953 at 1/100 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining in human lung cancer (A) while weak or negative staining in its adjacent noncancerous tissue (B) (PMID: 24465716). The section was incubated with ab252953 for 20 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab252953).

  • Anti-Aldolase antibody [EPR23181-39] - BSA and Azide free (ab273636)
    Anti-Aldolase antibody [EPR23181-39] - BSA and Azide free (ab273636)

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