Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-ME2 antibody [EPR7218] - BSA and Azide free (ab248127)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR7218] to ME2 - BSA and Azide free
  • Suitable for: WB, Flow Cyt, IHC-P, ICC
  • Reacts with: Human

Overview

  • Product name

    Anti-ME2 antibody [EPR7218] - BSA and Azide free
    See all ME2 primary antibodies

  • Description

    Rabbit monoclonal [EPR7218] to ME2 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, Flow Cyt, IHC-P, ICCmore details

  • Species reactivity

    Reacts with: Human
    Does not react with: Rat

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    ab248127 is the carrier-free version of ab126616 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    Ab248127 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Mouse: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-ME2 antibody [EPR7218] - BSA and Azide free (ab248127)
    Western blot - Anti-ME2 antibody [EPR7218] - BSA and Azide free (ab248127)
    All lanes : Anti-ME2 antibody [EPR7218] (ab126616) at 1/5000 dilution (purified)

    Lane 1 : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysates
    Lane 2 : 293T (Human embryonic kidney epithelial cell) whole cell lysates

    Lysates/proteins at 15 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 65 kDa



    This data was developed using ab126616, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

  • Flow Cytometry - Anti-ME2 antibody [EPR7218] - BSA and Azide free (ab248127)
    Flow Cytometry - Anti-ME2 antibody [EPR7218] - BSA and Azide free (ab248127)

    This data was developed using ab126616, the same antibody clone in a different buffer formulation.

    Flow Cytometry analysis of Jurkat (Human T cell leukemia T lymphocyte) cells labeling ME2 with purified ab126616 at 1:150 dilution (10 ug/ml) (red). Cells were fixed with 4% Paraformaldehyde and permeabilized with 90% Methanol. A Goat anti rabbit IgG (Alexa Fluor® 488) secondary antibody was used at 1:2000 dilution. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).
  • Immunocytochemistry - Anti-ME2 antibody [EPR7218] - BSA and Azide free (ab248127)
    Immunocytochemistry - Anti-ME2 antibody [EPR7218] - BSA and Azide free (ab248127)
    This data was developed using ab126616, the same antibody clone in a different buffer formulation.Immunocytochemistry/ Immunofluorescence analysis of HeLa (Human cervix adenocarcinoma epithelial cell) cells labeling ME2 with purified ab126616 at 1:150 dilution (9.1μg/ml). Cells were fixed in 100% Methanol. ab150077 Goat anti rabbit IgG(Alexa Fluor® 488) was used as the secondary antibody at 1:1000 dilution. DAPI nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ME2 antibody [EPR7218] - BSA and Azide free (ab248127)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ME2 antibody [EPR7218] - BSA and Azide free (ab248127)
    This data was developed using ab126616, the same antibody clone in a different buffer formulation.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human colon tissue sections labeling Me2 with Purified ab126616 at 1:1000 dilution (1.4 μg/ml). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). Tissue was counterstained with Hematoxylin. ImmunoHistoProbe one step HRP Polymer (ready to use) secondary antibody was used at 1:0 dilution. PBS instead of the primary antibody was used as the negative control.
  • Western blot - Anti-ME2 antibody [EPR7218] - BSA and Azide free (ab248127)
    Western blot - Anti-ME2 antibody [EPR7218] - BSA and Azide free (ab248127)
    All lanes : Anti-ME2 antibody [EPR7218] (ab126616) at 1/1000 dilution (unpurified)

    Lane 1 : 293T (Human embryonic kidney epithelial cell) cell lysate
    Lane 2 : Jurkat cell lysate
    Lane 3 : HeLa cell lysate
    Lane 4 : K562 cell lysate
    Lane 5 : MOLT4 cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-rabbit HRP at 1/2000 dilution

    Predicted band size: 65 kDa



    This data was developed using ab126616, the same antibody clone in a different buffer formulation.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ME2 antibody [EPR7218] - BSA and Azide free (ab248127)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ME2 antibody [EPR7218] - BSA and Azide free (ab248127)
    This data was developed using ab126616, the same antibody clone in a different buffer formulation.Unpurified ab126616, at 1/50 dilution, staining ME2 in Formalin-fixed, Paraffin-embedded Human colon tissue by Immunohistochemistry. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
  • Immunocytochemistry - Anti-ME2 antibody [EPR7218] - BSA and Azide free (ab248127)
    Immunocytochemistry - Anti-ME2 antibody [EPR7218] - BSA and Azide free (ab248127)
    This data was developed using ab126616, the same antibody clone in a different buffer formulation.Unpurified ab126616, at 1/100 dilution, staining ME2 in HeLa cells by Immunofluorescence.
  • Anti-ME2 antibody [EPR7218] - BSA and Azide free (ab248127)
    Anti-ME2 antibody [EPR7218] - BSA and Azide free (ab248127)

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