Mouse MDC ELISA Kit (Macrophage Derived Chemokine) (ab193726)
Key features and details
- Sensitivity: 2 pg/ml
- Range: 2 pg/ml - 600 pg/ml
- Sample type: Cell culture supernatant, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Mouse
Overview
-
Product name
Mouse MDC ELISA Kit (Macrophage Derived Chemokine)
See all MDC kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Overall Inter-assay Sample n Mean SD CV% Overall -
Sample type
Cell culture supernatant, Serum, Plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
2 pg/ml -
Range
2 pg/ml - 600 pg/ml -
Recovery
Sample specific recovery Sample type Average % Range Serum 108.6 103% - 117% Plasma 103 94% - 113% Cell culture media 112.7 93% - 138% -
Assay duration
One step assay -
Species reactivity
Reacts with: Mouse -
Product overview
Abcam's MDC (Macrophage Derived Chemokine) Mouse ELISA Kit (ab193726) is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of mouse MDC in serum, plasma and cell culture supernatants.
This assay employs an antibody specific for mouse MDC coated onto a 96-well plate. Standards and samples are pipetted into the wells and the immobilized antibody captures MDC present in the sample. The wells are washed and a biotinylated anti-mouse MDC antibody is added. After washing away any unbound biotinylated antibody, an HRP-conjugated streptavidin is pipetted into the wells. After incubation, the wells are again washed, followed by the addition of a TMB substrate solution to the wells. Color will develop in proportion to the amount of MDC bound in each well. Addition of the Stop Solution will change the color from blue to yellow, and the intensity of the color is measured at 450 nm.
-
Platform
Microplate (12 x 8 well strips)
Properties
-
Storage instructions
Store at -20°C. Please refer to protocols. -
Components 1 x 96 tests 20X Wash Buffer Concentrate 1 x 25ml 5X Assay Diluent B 1 x 15ml 500X HRP-Streptavidin Concentrate 1 x 200µl Assay Diluent A 1 x 30ml Biotinylated mouse MDC detection antibody (lyophilized) 2 vials mouse MDC Standards (lyophilized) 2 vials Pre-coated mouse MDC Microplate (12 strips x 8 wells) 1 unit Stop Solution 1 x 8ml TMB One-Step Substrate Reagent 1 x 12ml -
Research areas
-
Function
May play a role in the trafficking of activated/effector T-lymphocytes to inflammatory sites and other aspects of activated T-lymphocyte physiology. Chemotactic for monocytes, dendritic cells and natural killer cells. Mild chemoattractant for primary activated T-lymphocytes and a potent chemoattractant for chronically activated T-lymphocytes but has no chemoattractant activity for neutrophils, eosinophils, and resting T-lymphocytes. Binds to CCR4. Processed forms MDC(3-69), MDC(5-69) and MDC(7-69) seem not be active. -
Tissue specificity
Highly expressed in macrophage and in monocyte-derived dendritic cells, and thymus. Also found in lymph node, appendix, activated monocytes, resting and activated macrophages. Lower expression in lung and spleen. Very weak expression in small intestine. In lymph node expressed in a mature subset of Langerhans' cells (CD1a+ and CD83+). Expressed in Langerhans' cell histiocytosis but not in dermatopathic lymphadenopathy. Expressed in atopic dermatitis, allergic contact dermatitis skin, and psoriasis, in both the epidermis and dermis. -
Sequence similarities
Belongs to the intercrine beta (chemokine CC) family. -
Post-translational
modificationsThe N-terminal processed forms MDC(3-69), MDC(5-69) and MDC(7-69) are produced by proteolytic cleavage after secretion from monocyte derived dendrocytes. -
Cellular localization
Secreted. - Information by UniProt
-
Alternative names
- A 152E5.1
- ABCD 1
- ABCD1
see all -
Database links
- Entrez Gene: 20299 Mouse
- SwissProt: O88430 Mouse
- Unigene: 12895 Mouse
Images
-
Example of typical Mouse MDC standard curve using Assay dilutant A.
Data provided for demonstration purposes only. A new standard curve must be generated for each assay performed.
-
Example of typical Mouse MDC standard curve using Assay dilutant B.Data provided for demonstration purposes only. A new standard curve must be generated for each assay performed.
