Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-CCL27 antibody [EPR8583(2)] (ab194354)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR8583(2)] to CCL27
  • Suitable for: WB, ICC/IF
  • Reacts with: Human

Overview

  • Product name

    Anti-CCL27 antibody [EPR8583(2)]
    See all CCL27 primary antibodies

  • Description

    Rabbit monoclonal [EPR8583(2)] to CCL27

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    ICC/IF
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • Human CCL27 Recombinant protein; A431 cells.

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR8583(2)

  • Isotype

    IgG

  • Research areas

Images

  • Western blot - Anti-CCL27 antibody [EPR8583(2)] (ab194354)
    Western blot - Anti-CCL27 antibody [EPR8583(2)] (ab194354)
    Anti-CCL27 antibody [EPR8583(2)] (ab194354) at 1/20000 dilution + Human CCL27 Recombinant protein at 0.01 µg

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 13 kDa
    Observed band size: 13 kDa



    Blocking/Dilution buffer: 5% NFDM/TBST.

  • Immunocytochemistry/ Immunofluorescence - Anti-CCL27 antibody [EPR8583(2)] (ab194354)
    Immunocytochemistry/ Immunofluorescence - Anti-CCL27 antibody [EPR8583(2)] (ab194354)

    Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized A431 cells labeling CCL27 with ab194354 at 1/50 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/400 dilution. The nuclear counter stain is DAPI (blue).

  • Anti-CCL27 antibody [EPR8583(2)] (ab194354)
    Anti-CCL27 antibody [EPR8583(2)] (ab194354)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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