Anti-D4 GDI antibody [EPR14212(B)] (ab181252)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR14212(B)] to D4 GDI
- Suitable for: Flow Cyt, IHC-P, WB, ICC/IF
- Reacts with: Human
Overview
-
Product name
Anti-D4 GDI antibody [EPR14212(B)]
See all D4 GDI primary antibodies -
Description
Rabbit monoclonal [EPR14212(B)] to D4 GDI -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species Flow Cyt HumanICC/IF HumanIHC-P HumanWB Human -
Immunogen
This information is proprietary to Abcam and/or its suppliers.
-
Positive control
- Jurkat, U937, HL60 and Human spleen lysates; Human tonsil and Human bone marrow tissues; HL60 cells.
-
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 59% PBS, 0.05% BSA -
Concentration information loading...
-
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR14212(B) -
Isotype
IgG -
Research areas
Images
-
All lanes : Anti-D4 GDI antibody [EPR14212(B)] (ab181252) at 1/10000 dilution
Lane 1 : Jurkat lysate
Lane 2 : U937 lysate
Lane 3 : HL60 lysate
Lane 4 : Human spleen lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 22 kDa
Additional bands at: 23 kDa. We are unsure as to the identity of these extra bands.
-
Immunocytochemistry/ Immunofluorescence - Anti-D4 GDI antibody [EPR14212(B)] (ab181252) Image is courtesy of an Abreview submitted by Dr Armen Petrosyan.
Immunocytochemical immunoflurescence analysis of formaldehyde-fixed HEPG-2 cells, labelling D4 GDI with ab181252 at a dilution of 1/50 incubated for 3 hours at 22°C in 1% donkey serum in 0.1% PBST. Permeablization was with 0.2% Triton X-100. Blocking was with 1% donkey serum in 0.1% PBST for 1 hour at 22°C. Secondary used was a Donkey anti-rabbit polyclonal Alexa Fluor® 594 conjugate used at 1/200.
-
Flow Cytometrical analysis of HL60 cells labeling D4 GDI with ab181252 at 1/70 (red) compared to a negative control antibody (green).
-
Immunofluorescent analysis of HL60 cells labeling D4 GDI with ab181252 at 1/100 (green) with Goat anti rabbit IgG (Alexa Fluor®488) at 1/200 and DAPI staining (blue).
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-D4 GDI antibody [EPR14212(B)] (ab181252)
Immunohistochemical analysis of paraffin embedded Human bone marrow tissue labeling D4 GDI with ab181252 at 1/500.
Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-D4 GDI antibody [EPR14212(B)] (ab181252)
Immunohistochemical analysis of paraffin embedded Human tonsil tissue labeling D4 GDI with ab181252 at 1/500.
Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.
-