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Signal Transduction Cytoskeleton / ECM Cytoskeleton Microfilaments Actin etc Actin Assembly

Anti-ROCK1 antibody (ab245368)

Anti-ROCK1 antibody (ab245368)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Rabbit polyclonal to ROCK1
  • Suitable for: IP
  • Reacts with: Human
  • Isotype: IgG

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Overview

  • Product name

    Anti-ROCK1 antibody
    See all ROCK1 primary antibodies
  • Description

    Rabbit polyclonal to ROCK1
  • Host species

    Rabbit
  • Tested Applications & Species

    Application Species
    IP
    Human
    See all applications and species data
  • Immunogen

    Synthetic peptide within Human ROCK1 aa 400-450. The exact sequence is proprietary.
    Database link: Q13464

  • Positive control

    • IP: HeLa whole cell lysate.
  • General notes

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7
    Preservative: 0.09% Sodium azide
    Constituent: Tris citrate/phosphate

    pH 7 to 8
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    ab245368 was affinity purified using an epitope specific to ROCK1 immobilized on solid support.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Signal Transduction
    • Cytoskeleton / ECM
    • Cytoskeleton
    • Microfilaments
    • Actin etc
    • Actin Assembly
    • Signal Transduction
    • Protein Phosphorylation
    • Ser / Thr Kinases
    • Other Kinases
    • Cell Biology
    • Cell Cycle
    • Cell Division
    • Centromere
    • Cancer
    • Cell cycle
    • Cell division

Images

  • Immunoprecipitation - Anti-ROCK1 antibody (ab245368)
    Immunoprecipitation - Anti-ROCK1 antibody (ab245368)

    ROCK1 was immunoprecipitated from HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate (1 mg for IP, 25% of IP loaded) with ab245368 at 3 µg/mg lysate. Western blot was performed from the immunoprecipitate using a different Anti-ROCK1 antibody at 1 µg/ml (a different antibody was used for WB of each pair of lanes).

    Lane 1: ab245368 IP in HeLa whole cell lysate.
    Lane 2: Control IgG IP in HeLa whole cell lysate.

    Detection: Chemiluminescence with exposure time of 10-30 seconds.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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    Anti-ROCK2 + ROCK1 antibody [EP786Y] (ab45171)

    Applications: Flow Cyt, ICC/IF, IHC-P, IP, WB

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    Anti-ROCK1 antibody [EPR638Y] (ab134181)

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    Anti-ROCK2 + ROCK1 antibody [EP786Y] - BSA and Azide free (ab219587)

    Applications: Flow Cyt, ICC/IF, IHC-P, IP, WB

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    Alexa Fluor® 488 Anti-ROCK2 + ROCK1 antibody [EP786Y] (ab205432)

    Applications: ICC/IF

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    Anti-ROCK1 antibody [EPR638Y] - BSA and Azide free (ab230799)

    Applications: IHC-P, IP, WB

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