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Signal Transduction Cytoskeleton / ECM Cytoskeleton Microfilaments Actin etc Actin Assembly

Anti-PDPK1 (phospho S241) antibody (ab131098)

Anti-PDPK1 (phospho S241) antibody (ab131098)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Rabbit polyclonal to PDPK1 (phospho S241)
  • Suitable for: WB, IHC-P, ICC/IF
  • Reacts with: Rat, Human
  • Isotype: IgG

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Overview

  • Product name

    Anti-PDPK1 (phospho S241) antibody
    See all PDPK1 primary antibodies
  • Description

    Rabbit polyclonal to PDPK1 (phospho S241)
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity

    Reacts with: Rat, Human
  • Immunogen

    Synthetic phosphopeptide conjugated to KLH, corresponding to a region around phosphorylation site Serine 241 (

    A-N-S(p)-F-V

    ) of Human PDPK1 (NP_002604.1; UniProt O15530).
    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • Positive control

    • Extracts from 293 and PC12 cell treated with EGF; HeLa cells, Human breast carcinoma tissue
  • General notes

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Storage buffer

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituents: 49% PBS, 50% Glycerol, 0.88% Sodium chloride

    PBS is without Mg2+ and Ca2+
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    ab131098 was purified by affinity chromatography using epitope specific phosphopetide. Non phospho specific antibodies were removed by chromatography using non phosphopetide.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Signal Transduction
    • Cytoskeleton / ECM
    • Cytoskeleton
    • Microfilaments
    • Actin etc
    • Actin Assembly
    • Signal Transduction
    • Protein Phosphorylation
    • Ser / Thr Kinases
    • Other Kinases

Images

  • Western blot - Anti-PDPK1 (phospho S241) antibody (ab131098)
    Western blot - Anti-PDPK1 (phospho S241) antibody (ab131098)
    All lanes : Anti-PDPK1 (phospho S241) antibody (ab131098) at 1/500 dilution

    Lane 1 : 293 cell extract, untreated
    Lane 2 : 293 cell extract treated with EGF
    Lane 3 : PC12 cell extract, untreated
    Lane 4 : PC12 cell extract treated with EGF

    Predicted band size: 63 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDPK1 (phospho S241) antibody (ab131098)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDPK1 (phospho S241) antibody (ab131098)
    Immunohistochemical analysis of PDPK1 (phospho S241) in paraffin embedded Human breast carcinoma tissue, using ab131098 at a dilution of 1/50. Image on the right was preincubated with blocking peptide.
  • Immunocytochemistry/ Immunofluorescence - Anti-PDPK1 (phospho S241) antibody (ab131098)
    Immunocytochemistry/ Immunofluorescence - Anti-PDPK1 (phospho S241) antibody (ab131098)
    Immunofluorescence analysis of PDPK1 (phospho S241) in methanol fixed HeLa cells, using ab131098 at a dilution of 1/100.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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